Results 71 to 80 of about 55,424 (301)

Promiscuity‐Guided Enzyme Evolution via Substrate Multiplexed Screening

Angewandte Chemie, EarlyView.
Through historical context and recent examples, this Minireview summarizes the strengths, limitations, and practical considerations for the implementation of substrate multiplexed screening (SUMS) in enzyme evolution. ABSTRACT An enzyme's ability to react with diverse substrates while maintaining high yield and selectivity, termed “substrate ...
Holly A. Weilbaker, Meghan E. Campbell, Peyton M. Higgins, Andrew R. Buller   +3 more
wiley   +2 more sources

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

Novel Insights into Enzymatic Thermostability: The “Short Board” Theory and Zero‐Shot Hamiltonian Model

Advanced Science
Understanding the mechanism underlying thermostabilization in naturally stable enzymes and enhancing the thermostability of unstable enzymes are crucial aspects in enzyme engineering.
Min Liao, Shihao Feng, Xiaoqing Liu, Guoshun Xu, Sicong Li, Yingguo Bai, Huiying Luo, Bin Yao, Haobo Wang, Tao Tu   +9 more
doaj   +1 more source

FARS2 mutations presenting with pure spastic paraplegia and lesions of the dentate nuclei. [PDF]

, 2018
Mutations in FARS2, the gene encoding the mitochondrial phenylalanine-tRNA synthetase (mtPheRS), have been linked to a range of phenotypes including epileptic encephalopathy, developmental delay, and motor dysfunction.
Au, Margaret G, Graham, John M, Ibba, Michael, Pierson, Tyler M, Sahai, Supreet K, Salamon, Noriko, Steiner, Rebecca E   +6 more
core   +2 more sources

A Wood Plasticine With Controlled Phase‐Change Behavior and Malleability for Energy‐Closed‐Loop and Conformally Adaptive Thermal Management

Advanced Science, EarlyView.
A photothermal wood plasticine exhibiting controlled phase‐change behavior and malleable property is developed. The developed wood plasticine can provide all‐day, on‐demand, and low‐loss solar heating for geometrically diverse objects, including thermoelectric generator, human body, and battery, through its controlled crystallization‐melting ...
Jiazuo Zhou, Yifan Liu, Lei Qiao, Yuehe Gu, Taikun Yao, Wenbo Zhang, Fangmiao Wang, Yudong Li, Xinyao Ji, Lingyi Kong, Haiyue Yang, Yao Xiao, Chengyu Wang   +12 more
wiley   +1 more source

Robust and Thermally Stable Silicone Aerogels with Hyperconnected Network via Kinetically Optimized Hyperbranched Silane Precursors

Advanced Science, EarlyView.
Reactivity‐Programmed Assembly: A kinetic‐control strategy is reported for constructing hyperconnected silicone aerogels with a robust “thick‐neck” architecture. By exploiting the reactivity disparity of precursors, flexible segments are uniformly embedded within a rigid skeleton.
Aoqing Yan, Guixiang Li, Yaolan Li, Yi Luo, Bin Liu, Zhe Su, Hao Tian, Wei Shi, Bo Niu, Donghui Long   +9 more
wiley   +1 more source

Optimizing enzyme thermostability by combining multiple mutations using protein language model

mLife
Optimizing enzyme thermostability is essential for advancements in protein science and industrial applications. Currently, (semi‐)rational design and random mutagenesis methods can accurately identify single‐point mutations that enhance enzyme ...
Jiahao Bian, Pan Tan, Ting Nie, Liang Hong, Guang‐Yu Yang   +4 more
doaj   +1 more source

Gas Atmosphere Formed in Casting by Full Mold Process

Archives of Metallurgy and Materials, 2014
A test mold was designed to investigate the full mold process. Pressure changes in a gap developing between the pattern made of foamed polystyrene and the raising column of liquid alloy were determined. Studies were conducted pouring molds with cast iron
Mocek J., Chojecki A.
doaj   +1 more source

Rubredoxin Variant Folds without Iron [PDF]

, 1999
Pyroccocus furiosus rubredoxin (PFRD), like most studied hyperthermophilic proteins, does not undergo reversible folding. The irreversibility of folding is thought to involve PFRD’s iron-binding site.
Mayo, Stephen L., Strop, Pavel
core   +1 more source

Mechanozyme: An Artificial Enzyme With a Mechanophore Framework

Advanced Science, EarlyView.
Mechanical destabilization of a G‐quadruplex mechanophore in a DNAzyme markedly boosts its catalytic activity, demonstrating “mechanozymes” as artificial enzymes whose functions are modulated by mechanical stress exerted by ultrasonication. This mechanozyme principle is broadly applicable to control biomolecular activities.
Jiahao Ji, Pravin Pokhrel, Sajan Shakya, Bishal Pokhrel, Grinsun Sharma, Pratiksha Chaudhary, Hao Shen, Hanbin Mao   +7 more
wiley   +1 more source