Results 91 to 100 of about 38,411 (253)

A Biomimetic Solvatochromic Chip With Optimized Chemical Diversity for On‐Site Liquid Identification

Advanced Science, EarlyView.
A compact biomimetic photonic‐gustatory chip uses Hansen‐solubility‐parameter‐guided solvatochromic disks to create distinct color patterns in organic liquids. An optimized four‐disk array distinguishes solvent types, ratios, homologues, isomers, and alcoholic beverages. Coupled with smartphone imaging and deep learning, it identifies 80 liquid classes
Jintao Zhang, Hao Ouyang, Yin Cheng, Nuoye Xiong, Xunchi Ma, Xueling Li, Guangming Zhu, Liang Zhang, Jing Sun, Ranran Wang   +9 more
wiley   +1 more source

Attaining protein thermostability : A rationalized approach [PDF]

, 2016
Supervisors: Sanjukta Patra & Vishal TrivediIn proteins natural mutation occurs such that it tries to maintain maximum stability while retaining functionality indicating that nature may be rationalizing the way of attaining such mutations.
Chakravorty, Debamitra
core   +1 more source

AS‐pHopt: An Optimal pH Prediction Model Enhanced by Active Site of Enzymes

Advanced Intelligent Discovery, EarlyView.
To address the low accuracy of enzyme optimal pH (pHopt) prediction, this study develops active site‐based pHopt (AS‐pHopt), a prediction model enhanced by active site information and pseudo‐label prediction. Integrating key structural and physicochemical features affecting enzyme pHopt, AS‐pHopt uses Evolutionary Scale Modeling (ESM)‐2 with active ...
Wenxiang Song, Xinmin Li, Hongbo Yu, Le Xiong, Guixia Liu, Weihua Li, Yun Tang   +6 more
wiley   +1 more source

Firefly Luciferase Mutant with Enhanced Activity and Thermostability [PDF]

, 2018
The luciferase isolated from the firefly Photinus pyralis (Ppy) catalyzes a two-step reaction that results in the oxidation of d-luciferin accompanied by emission of yellow–green light with a peak at 560 nm.
Tania Pozzo (4913785), Yoko Nomura (318252), Farhima Akter, Louie, Angelique Y, Pozzo, Tania, Yohei Yokobayashi, Louie, Angelique Y., Angelique Y. Louie (240149), Yoko Nomura, Farhima Akter (780116), Yohei Yokobayashi (1304439), Nomura, Yoko, Yokobayashi, Yohei, Tania Pozzo, Akter, Farhima, Angelique Y. Louie   +15 more
core   +2 more sources

Thermostability engineering of industrial enzymes through structure modification

, 2022
Thermostability is an essential requirement of enzymes in the industrial processes to catalyze the reactions at high temperatures; thus, enzyme engineering through directed evolution, semi-rational design and rational design are commonly employed to ...
Nezhad, Nima Ghahremani, M. Normi, Yahaya, Raja Abd Rahman, Raja Noor Zaliha, Mohd Shariff, Fairolniza, Leow, Adam Thean Chor, Oslan, Siti Nurbaya   +5 more
core   +1 more source

Synthetic Particulate Subunit Vaccines Co‐Delivering Multiple Antigens Induce Protective Immunity Against Melioidosis

Advanced NanoBiomed Research, EarlyView.
Engineered endotoxin‐free Escherichia coli were used to assemble poly(3‐hydroxybutyrate) (PHB) biopolymer particles displaying multiple conserved Burkholderia pseudomallei antigens via PhaC‐mediated surface presentation. Following formulation with poly(I:C), the particulate vaccines induced antigen‐specific humoral and cellular immune responses ...
Md. Tanvir Kabir, Shuxiong Chen, Justine B. Bendo, Ashley Johnson, Aleksandra W. Debowski, Mitali Sarkar‐Tyson, Bernd H. A. Rehm   +6 more
wiley   +1 more source

Structural Study of Thermostable Ginsenoside β-Glucosidase from Caldicellulosiruptor bescii

Crystals
Protopanaxadiol-type ginsenosides, the major bioactive components of Panax ginseng, exhibit diverse pharmacological activities, but suffer from low oral bioavailability due to poor water solubility and membrane permeability. Enzymatic deglycosylation has
Jung-Min Choi
doaj   +1 more source

Toward enhancement of antibody thermostability and affinity by computational design in the absence of antigen

mAbs
Over the past two decades, therapeutic antibodies have emerged as a rapidly expanding domain within the field of biologics. In silico tools that can streamline the process of antibody discovery and optimization are critical to support a pipeline that is ...
Mark Hutchinson, Jeffrey A. Ruffolo, Nantaporn Haskins, Michael Iannotti, Giuliana Vozza, Tony Pham, Nurjahan Mehzabeen, Harini Shandilya, Keith Rickert, Rebecca Croasdale-Wood, Melissa Damschroder, Ying Fu, Andrew Dippel, Jeffrey J. Gray, Gilad Kaplan   +14 more
doaj   +1 more source

Design of mutants for enhanced thermostability of beta-glycosidase BglY from Thermus thermophilus

, 2013
Three design strategies, based on rational and semi-rational approaches, were employed to investigate the functional impact of thermostability-related amino acid substitutions in the beta-glycosidase BglY from Thermus thermophilus.
Zhang, SB (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, Chengdu 610041, Peoples R China, Wu, Zhong-Liu, Yi, Zhuo-Lin, Zhang, Shuai-Bing, Pei, Xiao-Qiong   +4 more
core  

A Strategy for Simultaneous Engineering of Interspecies Cross-Reactivity, Thermostability, and Expression of a Bispecific 5T4 x CD3 DART^® Molecule for Treatment of Solid Tumors

Antibodies
Background: Bispecific antibodies represent a promising class of biologics for cancer treatment. However, their dual specificity and complex structure pose challenges in the engineering process, often resulting in molecules with good functional but poor ...
Renhua R. Huang, Michael Spliedt, Tom Kaufman, Sergey Gorlatov, Bhaswati Barat, Kalpana Shah, Jeffrey Gill, Kurt Stahl, Jennifer DiChiara, Qian Wang, Jonathan C. Li, Ralph Alderson, Paul A. Moore, Jennifer G. Brown, James Tamura, Xiaoyu Zhang, Ezio Bonvini, Gundo Diedrich   +17 more
doaj   +1 more source