Results 101 to 110 of about 55,424 (301)

Cold Adaptation of a Mesophilic Subtilisin-like Protease by Laboratory Evolution [PDF]

open access: yes, 2000
Enzymes isolated from organisms native to cold environments generally exhibit higher catalytic efficiency at low temperatures and greater thermosensitivity than their mesophilic counterparts.
Arnold, Frances H.   +2 more
core   +1 more source

An Idea to Explore: From How to Why: Using Evolutionary Biochemistry to Inspire Biochemistry Education

open access: yesBiochemistry and Molecular Biology Education, EarlyView.
ABSTRACT Traditional biochemistry instruction often emphasizes mechanistic detail, that is, how molecules and pathways function, without equally addressing why they have their present forms. This fact‐centered approach can leave students overwhelmed and disconnected from the broader scientific narrative.
Alberto Vázquez‐Salazar
wiley   +1 more source

Engineering Spin State of Ni Single‐Atoms to Enhance Electrocatalytic Activity of Sulfur Conversion in Lithium–Sulfur Batteries

open access: yesCarbon Energy, EarlyView.
Ni SACs with high‐spin state show strong catalytic activity toward lithium polysulfides (LPSs), whereas the Ni SACs with low‐spin state have strong adsorption capacity for LPSs. Therefore, the preparation of Ni SACs with an intermediate spin state by controlling pyridinic N and pyrrolic N enables an excellent balance between the adsorption and ...
Mengyang Li   +16 more
wiley   +1 more source

Structural Study of Thermostable Ginsenoside β-Glucosidase from Caldicellulosiruptor bescii

open access: yesCrystals
Protopanaxadiol-type ginsenosides, the major bioactive components of Panax ginseng, exhibit diverse pharmacological activities, but suffer from low oral bioavailability due to poor water solubility and membrane permeability. Enzymatic deglycosylation has
Jung-Min Choi
doaj   +1 more source

Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes. [PDF]

open access: yes, 2012
BAckground: Massively parallel sequencing technology is revolutionizing approaches to genomic and genetic research. Since its advent, the scale and efficiency of Next-Generation Sequencing (NGS) has rapidly improved. In spite of this success, sequencing
Campino, Susana   +10 more
core  

Dynamic Control of Nucleic Acids Self‐Assembly and Expression Using Photoswitches

open access: yesChemistry – A European Journal, EarlyView.
We review here the recent progress made in the design of molecular photoswitches, and highlight their implementation for the dynamic control over nucleic acids self‐assembly and expression. ABSTRACT Synthetic nucleic acids have become readily available and now constitute versatile building blocks in materials science—where they can be used to engineer ...
Noemí Nogal   +4 more
wiley   +1 more source

Toward enhancement of antibody thermostability and affinity by computational design in the absence of antigen

open access: yesmAbs
Over the past two decades, therapeutic antibodies have emerged as a rapidly expanding domain within the field of biologics. In silico tools that can streamline the process of antibody discovery and optimization are critical to support a pipeline that is ...
Mark Hutchinson   +14 more
doaj   +1 more source

Detection of genetically modified plant products by protein strip testing: an evaluation of real-life samples [PDF]

open access: yes, 2007
The determination of the presence of genetically modified plant material by the detection of expressed genetically engineered proteins using lateral flow protein strip tests has been evaluated in different matrices. The presence of five major genetically
CASI, AL   +7 more
core   +1 more source

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