Protein engineering of Pseudomonas fluorescens peroxidase Dyp1B for oxidation of phenolic and polymeric lignin substrates [PDF]
, 2019 Directed evolution was applied to dye-decolourizing peroxidase Dyp1B from Pseudomonas fluorescens Pf-5, in order to enhance the activity for oxidation of phenolic and lignin substrates.
Ashley, Ben +6 more
core +2 more sources
Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120
BMC Biotechnology, 2018 Background Lipoxygenase (LOX) from Anabaena sp. PCC 7120 (Ana-rLOX) offers important applications in the food industry, especially for improving aroma and dough rheological properties.
Hui Qian +7 more
doaj +1 more source
Molecules, 2022 To extend the application range of L-asparaginase in food pre-processing, the thermostability improvement of the enzyme is essential. Herein, two non-conserved cysteine residues with easily oxidized free sulfhydryl groups, Cys8 and Cys283, of ...
Linshu Jiao +7 more
doaj +1 more source
A family of thermostable fungal cellulases created by structure-guided recombination [PDF]
, 2009 SCHEMA structure-guided recombination of 3 fungal class II cellobiohydrolases (CBH II cellulases) has yielded a collection of highly thermostable CBH II chimeras.
A. Villalobos +19 more
core +3 more sources
Functional stability of HIV-1 envelope trimer affects accessibility to broadly neutralizing antibodies at its apex [PDF]
, 2017 The trimeric envelope glycoprotein spike (Env) of HIV-1 is the target of vaccine development to elicit broadly neutralizing antibodies (bnAbs). Env trimer instability and heterogeneity in principle make subunit interfaces inconsistent targets for the ...
Gift, Syna Kuriakose +4 more
core +2 more sources
Structural Rigidity and Protein Thermostability in Variants of Lipase A from Bacillus subtilis. [PDF]
PLoS ONE, 2015 Understanding the origin of thermostability is of fundamental importance in protein biochemistry. Opposing views on increased or decreased structural rigidity of the folded state have been put forward in this context.
Prakash Chandra Rathi +2 more
doaj +1 more source
The structure of a tetrameric α-carbonic anhydrase fromThermovibrio ammonificansreveals a core formed around intermolecular disulfides that contribute to its thermostability [PDF]
, 2014 Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide to bicarbonate. A thermophilic Thermovibrio ammonificans α-carbonic anhydrase (TaCA) has been expressed in Escherichia coli and structurally and biochemically characterized ...
Berg, Svein +7 more
core +1 more source
Effects of mutations in the helix G region of horseradish peroxidase [PDF]
, 2008 Horseradish peroxidase (HRP) has long attracted intense research interest and is used in many biotechnological fields, including diagnostics, biosensors and biocatalysis.
Barry J. Ryan +59 more
core +1 more source
Frontiers in Bioengineering and Biotechnology, 2020 Lipases catalyze the hydrolysis of fats and oils, and have been widely used in various industrial fields. However, bacterial lipases have a lower thermostability in industrial processes, which was a limiting factor in their industrial application.
Lijun Guan +8 more
doaj +1 more source
Molecular Dynamics Analysis of Thermostable DNA Pol I ITB-1
Microbiology Indonesia, 2010 One of the thermostable enzymes, which has been widely used in the biotechnological research, is DNA polymerase. The coding sequence of local DNA Pol I gene from a local thermophilic bacterium, namely DNA Pol I ITB-1, has been cloned, sequenced, and ...
RUKMAN HERTADI +2 more
doaj +1 more source