Results 31 to 40 of about 162,084 (246)

Incorporation characteristics of exogenous 15N-labeled thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine into bacterial DNA.

PLoS ONE, 2020
Bacterial production has been often estimated from DNA synthesis rates by using tritium-labeled thymidine. Some bacteria species cannot incorporate extracellular thymidine into their DNA, suggesting their biomass production might be overlooked when using
Kenji Tsuchiya, Tomoharu Sano, Noriko Tomioka, Ayato Kohzu, Kazuhiro Komatsu, Ryuichiro Shinohara, Shinji Shimode, Tatsuki Toda, Akio Imai   +8 more
doaj   +1 more source

Preparation of Tritium-Labeled Thymidine [PDF]

, 1957
1) De la thymidine est marquée avec du tritium par échange avec les hydrogènes d'eau tritiée à 100°C pendant 24 heures en présence de catalyseur d'Adams réduit; 2) Les tritiums labiles sont éliminés à chaud dans une solution aqueuse de NaOH.
Verly, Walter, Hunebelle, G.
core   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Thymidine Metabolism in Neurospora crassa [PDF]

, 1968
The metabolism of thymidine by Neurospora crassa was investigated after radioactive labeling experiments showed that thymidine is not specifically incorporated into the DNA of Neurospora; a finding in contrast to the results of labeled thymidine ...
Williams, Larry Gale
core   +1 more source

Inactivation of the Thymidylate Synthase thyA in Non-typeable Haemophilus influenzae Modulates Antibiotic Resistance and Has a Strong Impact on Its Interplay with the Host Airways

Frontiers in Cellular and Infection Microbiology, 2017
Antibacterial treatment with cotrimoxazol (TxS), a combination of trimethoprim and sulfamethoxazole, generates resistance by, among others, acquisition of thymidine auxotrophy associated with mutations in the thymidylate synthase gene thyA, which can ...
Irene Rodríguez-Arce, Sara Martí, Sara Martí, Begoña Euba, Begoña Euba, Ariadna Fernández-Calvet, Javier Moleres, Nahikari López-López, Montserrat Barberán, José Ramos-Vivas, José Ramos-Vivas, Fe Tubau, Fe Tubau, Carmen Losa, Carmen Ardanuy, Carmen Ardanuy, José Leiva, José E. Yuste, José E. Yuste, Junkal Garmendia, Junkal Garmendia   +20 more
doaj   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Clinicopathology and Diagnosis Delay in a 40-Year-Old with Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE)

Case Reports in Gastroenterology, 2020
Mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) is an autosomal recessive and fatal multisystem metabolic disorder. It presents with wide-ranging gastrointestinal and neurologic symptoms.
Antonios Tawk, Mohammed Hussein Kamarreddine, Mona Dagher, Ghadi Abboud, Mohamad Chams, Fatmeh Ghandour-Hajj, Mounir Khoury, Said Farhat   +7 more
doaj   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Clinicopathological Significance of Thymidine Phosphorylase Expressed in Node-Negative Breast Cancer Tumors [PDF]

, 2001
The expression of thymidine phosphorylase (dThd Pase) in node-negative human invasive ductal carcinoma tissues was investigated by the immunocytochemical method using anti-thymidine phosphorylase monoclonal antibody.Subjects were 5 recurrent and 30 non ...
土屋, 眞一, 麻沼, 和彦, 本原, 昭子, 丸山, 正幸, 藤森, 実, 草間, 律, 天野, 純, 浜, 善久   +7 more
core  

Expression of Thymidine Phosphorylase in Lymph Nodes Involved with Mycosis Fungoides and Sézary Syndrome

Advances in Hematology, 2011
Thymidine phosphorylase may be overexpressed in both neoplastic cells and tumor stromal cells in a variety of malignancies. Our study explores thymidine phosphorylase expression in lymph nodes (LNs) from patients with mycosis fungoides (MF) or Sézary ...
Xingcao Nie, Rekha Bhat, Essel Dulaimi Al-Saleem, Eric C. Vonderheid, J. Steve Hou   +4 more
doaj   +1 more source