Results 61 to 70 of about 36,815 (253)
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Time-Resolved X-Ray Crystallography of Heme Proteins [PDF]
, 2008 Heme proteins, with their natural photosensitivity, are excellent systems for the application of time-resolved crystallographic methods. Ligand dissociation can be readily initiated by a short laser pulse with global structural changes probed at the atomic level by X-rays in real time.
Vukica, Srajer, William E, Royer
openaire +2 more sources
Towards time-resolved serial crystallography in a microfluidic device [PDF]
Acta Crystallographica Section F Structural Biology Communications, 2015 Serial methods for crystallography have the potential to enable dynamic structural studies of protein targets that have been resistant to single-crystal strategies. The use of serial data-collection strategies can circumvent challenges associated with radiation damage and repeated reaction initiation.
Ashtamurthy S, Pawate +9 more
openaire +2 more sources
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
Specific radiation damage is a lesser concern at room temperature
IUCrJ, 2019 Carrying out macromolecular crystallography (MX) experiments at cryogenic temperatures significantly slows the rate of global radiation damage, thus facilitating the solution of high-resolution crystal structures of macromolecules.
Guillaume Gotthard +5 more
doaj +1 more source
Creating Ti–Fe α/β Alloys by Diffusion‐Driven Solid‐State Processing
Advanced Engineering Materials, EarlyView.This study proposes making alloys containing fast diffusing elements that are difficult to produce by ingot metallurgy, by diffusion‐driven solid‐state HIP processing of elemental powders and low‐temperature homogenisation. Here, novel Fe‐Ti α–β alloys are formed having fine α–β lamellae, a small β prior grain size without significant intermetallics ...
Jiaqi Xu +10 more
wiley +1 more source
, 2021 Serial crystallography (SX) provides room-temperature crystal structures with minimal radiation damage and facilitates the comprehension of molecular dynamics through time-resolved studies.
Nam, Ki Hyun, Cho, Yunje
core +1 more source
Crystals, 2021 The review provides a summary of the current methods of tracing photoexcitation processes and structural dynamics in the solid state, putting major emphasis on the X-ray diffraction techniques (time-resolved Laue diffraction on synchrotron sources and ...
Krystyna A. Deresz +3 more
doaj +1 more source
Advanced Engineering Materials, EarlyView.Phase‐field simulations coupled with dislocation‐density‐based crystal plasticity modeling reproduce γ′ rafting behavior in single‐crystal Ni‐based superalloys under varied loading conditions. The model captures both macroscopic creep and microscopic morphology evolution, with results matching high‐temperature creep experiments.
Micheal Younan +5 more
wiley +1 more source
In crystallo observation of three metal ion promoted DNA polymerase misincorporation
Nature Communications, 2022 By observing DNA polymerase misincorporation with time-resolved crystallography, the authors visualize three-metal ion dependent polymerase catalysis and identify A-site metal-mediated primer alignment as a key step in nucleotide discrimination.
Caleb Chang, Christie Lee Luo, Yang Gao
doaj +1 more source