Results 51 to 60 of about 36,815 (253)

Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA [PDF]

, 2017
X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced ...
Kimura, T., Tono, Kensuke,, Richard Neutze, Naoya Miyauchi, Fumitaka Mafuné, Davidsson, Jan, Neutze, Richard, Jan Davidsson, Tanaka, Rie,, Kohno, Jun-ya,, Yoshihiro Takeda, Nango, Eriko, Takeda, Yoshihiro, Mafune, Fumitaka,, Hatsui, Takaki, Nomura, T., Murata, Michio,, Kohno, J., Tanaka, T., Murata, M., Miyajima, Ken, Jun-ya Kohno, Song, Changyong,, Miyajima, Ken,, Davidsson, J., Changyong Song, Takashi Nomura, Nango, E., Nakane, T., Tanaka, Rie, Joti, Yasumasa, Miyauchi, Naoya, Takanori Nakane, Nomura, Takashi, Nakane, Takanori,, Kensuke Tono, Yasumasa Joti, Hatsui, T., Hatsui, Takaki,, Kohno, Jun-ya, Iwata, So, Yabashi, Makina,, Joti, Y., Kameshima, Takashi, Makina Yabashi, Kameshima, Takashi,, Miyauchi, N., Tanaka, Tomoyuki,, Kimura, Tetsunari,, Nakane, Takanori, Takeda, Y., Nango, Eriko,, Eriko Nango, Takashi Kameshima, Ken Miyajima, Michio Murata, Kubo, Minoru,, Tono, K., Kubo, M., Song, C. Y., Tanaka, Tomoyuki, Owada, S., Owada, Shigeki, Kameshima, T., Mafune, F., Owada, Shigeki,, Iwata, So,, Tomoyuki Tanaka, Rie Tanaka, Nomura, Takashi,, Joti, Yasumasa,, Kimura, Tetsunari, Tanaka, R., Tetsunari Kimura, Iwata, S., Davidsson, Jan,, Takeda, Yoshihiro,, Miyauchi, Naoya,, Yabashi, Makina, Minoru Kubo, So Iwata, Song, Changyong, Shigeki Owada, Takaki Hatsui, Mafune, Fumitaka, Murata, Michio, Miyajima, K., Yabashi, M., Tono, Kensuke, Kubo, Minoru, Neutze, Richard,   +90 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow

IUCrJ, 2020
Serial crystallography has enabled the study of complex biological questions through the determination of biomolecular structures at room temperature using low X-ray doses.
Diana C. F. Monteiro, David von Stetten, Claudia Stohrer, Marta Sans, Arwen R. Pearson, Gianluca Santoni, Peter van der Linden, Martin Trebbin   +7 more
doaj   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Observing one-divalent-metal-ion-dependent and histidine-promoted His-Me family I-PpoI nuclease catalysis in crystallo

eLife
Metal-ion-dependent nucleases play crucial roles in cellular defense and biotechnological applications. Time-resolved crystallography has resolved catalytic details of metal-ion-dependent DNA hydrolysis and synthesis, uncovering the essential roles of ...
Caleb Chang, Grace Zhou, Yang Gao
doaj   +1 more source

Time-resolved β-lactam cleavage by L1 metallo-β-lactamase

Nature Communications, 2022
Metallo-β-lactamases cleave β-lactam moiety of many broadly used antibiotics. Here the authors captured mechanistic details of the enzyme catalyzed reaction using time-resolved xray synchrotron serial crystallography.
M. Wilamowski, D. A. Sherrell, Y. Kim, A. Lavens, R. W. Henning, K. Lazarski, A. Shigemoto, M. Endres, N. Maltseva, G. Babnigg, S. C. Burdette, V. Srajer, A. Joachimiak   +12 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Time-resolved serial synchrotron and serial femtosecond crystallography of heme proteins using photocaged nitric oxide

IUCrJ
Time-resolved X-ray crystallography is undergoing a renaissance due to the development of serial crystallography at synchrotron and XFEL beamlines. Crucial to such experiments are efficient and effective methods for uniformly initiating time-dependent ...
Peter Smyth, Sofia Jaho, Lewis J. Williams, Gabriel Karras, Ann Fitzpatrick, Amy J. Thompson, Sinan Battah, Danny Axford, Sam Horrell, Marina Lučić, Kotone Ishihara, Machika Kataoka, Hiroaki Matsuura, Kanji Shimba, Kensuke Tono, Takehiko Tosha, Hiroshi Sugimoto, Shigeki Owada, Michael A. Hough, Jonathan A.R. Worrall, Robin L. Owen   +20 more
doaj   +1 more source

Using synchrotrons and XFELs for time-resolved X-ray crystallography and solution scattering experiments on biomolecules [PDF]

, 2015
International audienceTime-resolved structural information is key to understand the mechanism of biological processes, such as catalysis and signalling.
Monteiro, Diana CF, Pearson, Arwen R, Yorke, BA, Cammarata, M, Cammarata, Marco, Yorke, Briony A, Monteiro, DCF, Levantino, Matteo, Pearson, AR   +8 more
core   +1 more source

A 2D smart pixel detector for time-resolved crystallography [PDF]

Review of Scientific Instruments, 1996
A smart pixel detector is being developed for time-resolved crystallography for biological and material science applications. Using the pixel detector presented here, the Laue method will enable the study of the evolution of structural changes that occur within a protein as a function of time.
Beuville, E., Cork, C.P., Earnest, T.N., Mar, W., Millaud, J., Nygren, D.R., Padmore, H.A., Turko, B.T., Zizka, G.J., Datte, P., Xuong, N.-H.   +10 more
openaire   +2 more sources