Results 31 to 40 of about 36,815 (253)

Minimized sample consumption for time-resolved serial crystallography applied to the redox cycle of human NQO1 [PDF]

Communications Chemistry
Sample consumption for serial femtosecond crystallography with X-ray free electron lasers remains a major limitation preventing broader use in macromolecular crystallography.
Diandra Doppler, Alice Grieco, Domin Koh, Abhik Manna, Adil Ansari, Roberto Alvarez, Konstantinos Karpos, Hung Le, Mukul Sonker, Gihan K. Ketawala, Samira Mahmud, Isabel Quereda-Moraleda, Sayantee Sen, Angel L. Pey, Romain Letrun, Katerina Dörner, Jayanath C. P. Koliyadu, Raphael de Wijn, Johan Bielecki, Huijong Han, Chan Kim, Faisal H. M. Koua, Adam Round, Abhisakh Sarma, Tokushi Sato, Christina Schmidt, Mohammad Vakili, Dmitrii Zabelskii, Richard Bean, Adrian P. Mancuso, Joachim Schulz, Raimund Fromme, Milagros Medina, Thomas D. Grant, Petra Fromme, Richard A. Kirian, Sabine Botha, Jose Manuel Martin-Garcia, Alexandra Ros   +38 more
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Revisiting the co-crystal structure of a DNA glycosylase with photocaged substrate: a suitable time-resolved crystallography target? [PDF]

IUCrJ
Co-crystal structures of a base-excision DNA-repair enzyme (human 8-oxoguanine DNA glycosylase; hOgg1) in complex with a photocaged 8-oxoguanine DNA lesion were determined before and after uncaging via illumination at 2.81 and 2.48 Å resolution ...
Tomoki Imura, Yuhei Hosokawa, Kai-Chun Yang, Yuki Ban, Hsuan-Yu Shih, Junpei Yamamoto, Manuel Maestre-Reyna   +6 more
doaj   +2 more sources

Mapping Enzyme Landscapes by Time-Resolved Crystallography with Synchrotron and X-Ray Free Electron Laser Light. [PDF]

Annu Rev Biophys, 2022
Wilson MA.
europepmc   +2 more sources

Capturing macromolecular dynamics using time-resolved serial crystallography at X- ray free electron lasers and synchrotron light sources [PDF]

Structural Dynamics
In recent years, time-resolved serial crystallography has emerged as a transformative technique for unraveling the intricate dynamics of macromolecules at atomic resolution.
Sandra Mous, Guillaume Gotthard, David Ehrenberg, Saumik Sen, Tobias Weinert, Philip Johnson, Daniel James, Karol Nass, Antonia Furrer, Pikyee Ma, Steffen Bruenle, Cecilia Casadei, Isabelle Martiel, Florian Dworkowski, Dardan Gashi, Petr Skopintsev, Maximilian Wranik, Gregor Knopp, Ezequiel Panepucci, Valerie Panneels, Claudio Cirelli, Dmitry Ozerov, Gebhard Schertler, Meitian Wang, Chris Milne, Joerg Standfuss, Igor Schapiro, Joachim Heberle, Przemyslaw Nogly   +28 more
doaj   +2 more sources

The time revolution in macromolecular crystallography

Structural Dynamics
Macromolecular crystallography has historically provided the atomic structures of proteins fundamental to cellular functions. However, the advent of cryo-electron microscopy for structure determination of large and increasingly smaller and flexible ...
Georgii Khusainov, Joerg Standfuss, Tobias Weinert   +2 more
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Photoswitch dissociation from a G protein-coupled receptor resolved by time-resolved serial crystallography

Nature Communications
G protein-coupled receptors (GPCRs) are the largest family of cell surface receptors in humans. The binding and dissociation of ligands tunes the inherent conformational flexibility of these important drug targets towards distinct functional states. Here
Hannah Glover, Torben Saßmannshausen, Quentin Bertrand, Matilde Trabuco, Chavdar Slavov, Arianna Bacchin, Fabio Andres, Yasushi Kondo, Robin Stipp, Maximilian Wranik, Georgii Khusainov, Melissa Carrillo, Demet Kekilli, Jie Nan, Ana Gonzalez, Robert Cheng, Werner Neidhart, Tobias Weinert, Filip Leonarski, Florian Dworkowski, Michal Kepa, Josef Wachtveitl, Michael Hennig, Joerg Standfuss   +23 more
doaj   +2 more sources

Time-resolved crystallography and protein design: signalling photoreceptors and optogenetics. [PDF]

Philos Trans R Soc Lond B Biol Sci, 2014
Moffat K.
europepmc   +2 more sources

Fixed target matrix for femtosecond time-resolved and in situ serial micro-crystallography

Structural Dynamics, 2015
We present a crystallography chip enabling in situ room temperature crystallography at microfocus synchrotron beamlines and X-ray free-electron laser (X-FEL) sources.
C. Mueller, A. Marx, S. W. Epp, Y. Zhong, A. Kuo, A. R. Balo, J. Soman, F. Schotte, H. T. Lemke, R. L. Owen, E. F. Pai, A. R. Pearson, J. S. Olson, P. A. Anfinrud, O. P. Ernst, R. J. Dwayne Miller   +15 more
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Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser. [PDF]

Nature, 2014
Kupitz C, Basu S, Grotjohann I, Fromme R, Zatsepin NA, Rendek KN, Hunter MS, Shoeman RL, White TA, Wang D, James D, Yang JH, Cobb DE, Reeder B, Sierra RG, Liu H, Barty A, Aquila AL, Deponte D, Kirian RA, Bari S, Bergkamp JJ, Beyerlein KR, Bogan MJ, Caleman C, Chao TC, Conrad CE, Davis KM, Fleckenstein H, Galli L, Hau-Riege SP, Kassemeyer S, Laksmono H, Liang M, Lomb L, Marchesini S, Martin AV, Messerschmidt M, Milathianaki D, Nass K, Ros A, Roy-Chowdhury S, Schmidt K, Seibert M, Steinbrener J, Stellato F, Yan L, Yoon C, Moore TA, Moore AL, Pushkar Y, Williams GJ, Boutet S, Doak RB, Weierstall U, Frank M, Chapman HN, Spence JC, Fromme P.   +58 more
europepmc   +2 more sources

Kilohertz serial crystallography with the JUNGFRAU detector at a fourth-generation synchrotron source

IUCrJ, 2023
Serial and time-resolved macromolecular crystallography are on the rise. However, beam time at X-ray free-electron lasers is limited and most third-generation synchrotron-based macromolecular crystallography beamlines do not offer the necessary ...
Filip Leonarski, Jie Nan, Zdenek Matej, Quentin Bertrand, Antonia Furrer, Ishkhan Gorgisyan, Monika Bjelčić, Michal Kepa, Hannah Glover, Viktoria Hinger, Thomas Eriksson, Aleksander Cehovin, Mikel Eguiraun, Piero Gasparotto, Aldo Mozzanica, Tobias Weinert, Ana Gonzalez, Jörg Standfuss, Meitian Wang, Thomas Ursby, Florian Dworkowski   +20 more
doaj   +1 more source