Results 21 to 30 of about 36,815 (253)

Appraising protein conformational changes by resampling time-resolved serial x-ray crystallography data [PDF]

Structural Dynamics
With the development of serial crystallography at both x-ray free electron laser and synchrotron radiation sources, time-resolved x-ray crystallography is increasingly being applied to study conformational changes in macromolecules.
Adams Vallejos, Gergely Katona, Richard Neutze   +2 more
doaj   +3 more sources

Spitrobot-2 advances time-resolved cryo-trapping crystallography to under 25 ms [PDF]

Communications Chemistry
We previously introduced the spitrobot, a protein crystal plunging system that enables reaction quenching via cryo-trapping with a time resolution in the millisecond range.
Maria Spiliopoulou, Caitlin E. Hatton, Martin Kollewe, Jan-Philipp Leimkohl, Hendrik Schikora, Friedjof Tellkamp, Pedram Mehrabi, Eike C. Schulz   +7 more
doaj   +3 more sources

Time-resolved crystallography using the Hadamard transform. [PDF]

Nat Methods, 2014
We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse.
Yorke BA, Beddard GS, Owen RL, Pearson AR.   +3 more
europepmc   +6 more sources

Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography [PDF]

Nature Communications, 2023
We introduce the spitrobot, a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, where the crystals ...
Pedram Mehrabi, Sihyun Sung, David von Stetten, Andreas Prester, Caitlin E. Hatton, Stephan Kleine-Döpke, Alexander Berkes, Gargi Gore, Jan-Philipp Leimkohl, Hendrik Schikora, Martin Kollewe, Holger Rohde, Matthias Wilmanns, Friedjof Tellkamp, Eike C. Schulz   +14 more
doaj   +2 more sources

Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy [PDF]

Nature Communications, 2020
rsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolution light microscopy. Here the authors present the structure of an rsEGFP2 ground-state intermediate after excited state-decay that was obtained by nanosecond time-resolved ...
Joyce Woodhouse, Gabriela Nass Kovacs, Nicolas Coquelle, Lucas M. Uriarte, Virgile Adam, Thomas R. M. Barends, Martin Byrdin, Eugenio de la Mora, R. Bruce Doak, Mikolaj Feliks, Martin Field, Franck Fieschi, Virginia Guillon, Stefan Jakobs, Yasumasa Joti, Pauline Macheboeuf, Koji Motomura, Karol Nass, Shigeki Owada, Christopher M. Roome, Cyril Ruckebusch, Giorgio Schirò, Robert L. Shoeman, Michel Thepaut, Tadashi Togashi, Kensuke Tono, Makina Yabashi, Marco Cammarata, Lutz Foucar, Dominique Bourgeois, Michel Sliwa, Jacques-Philippe Colletier, Ilme Schlichting, Martin Weik   +33 more
doaj   +2 more sources

Hemoprotein time‐resolved X‐ray crystallography [PDF]

IUBMB Life, 2008
AbstractIn the last decade the role of structural dynamics in controlling protein function was actively investigated using new and advanced experimental approaches. In particular, time resolved crystallography, despite some practical difficulties, is being used extensively to complement the study of protein structure‐function relationships with ...
M. Milani, M. Nardini, A. Pesce, E. Mastrangelo, M. Bolognesi   +4 more
openaire   +4 more sources

Filling data analysis gaps in time-resolved crystallography by machine learning [PDF]

Structural Dynamics
There is a growing understanding of the structural dynamics of biological molecules fueled by x-ray crystallography experiments. Time-resolved serial femtosecond crystallography (TR-SFX) with x-ray Free Electron Lasers allows the measurement of ultrafast
Justin Trujillo, Russell Fung, Madan Kumar Shankar, Peter Schwander, Ahmad Hosseinizadeh   +4 more
doaj   +2 more sources

Serial synchrotron crystallography for time-resolved structural biology [PDF]

Current Opinion in Structural Biology, 2020
The current state-of-the-art experiments in time-resolved structural biology are undoubtedly the recent extremely impressive results that are emerging from XFEL-based experiments. However, there is a large range of macromolecular systems where the biological interest is predominantly in the slower dynamics (μs-s), that produce well diffracting ...
Pearson, A., Mehrabi, P.
openaire   +4 more sources

Time-Resolved Single-Crystal X-Ray Crystallography [PDF]

, 2020
In this chapter the development of time-resolved crystallography is traced from its beginnings more than 30 years ago. The importance of being able to “watch” chemical processes as they occur rather than just being limited to three-dimensional pictures of the reactant and final product is emphasised, and time-resolved crystallography provides the ...
Paul R. Raithby, Raithby, Paul R.
openaire   +2 more sources

Analysis of the HindIII-catalyzed reaction by time-resolved crystallography. [PDF]

Acta Crystallogr D Biol Crystallogr, 2015
In order to investigate the mechanism of the reaction catalyzed by HindIII, structures of HindIII–DNA complexes with varying durations of soaking time in cryoprotectant buffer containing manganese ions were determined by the freeze-trap method. In the crystal structures of the complexes obtained after soaking for a longer duration, two manganese ions ...
Kawamura T, Kobayashi T, Watanabe N.
europepmc   +4 more sources