Results 1 to 10 of about 36,815 (253)

Slow protein dynamics probed by time-resolved oscillation crystallography at room temperature

IUCrJ, 2022
The development of serial crystallography over the last decade at XFELs and synchrotrons has produced a renaissance in room-temperature macromolecular crystallography (RT-MX), and fostered many technical and methodological breakthroughs designed to study
Sylvain Aumonier, Sylvain Engilberge, Nicolas Caramello, David von Stetten, Guillaume Gotthard, Gordon A. Leonard, Christoph Mueller-Dieckmann, Antoine Royant   +7 more
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Time‐resolved protein crystallography [PDF]

Protein Science, 1992
Advances in synchrotron radiation technology have allowed exposure times from protein crystals of the order of milliseconds to be used routinely, and in exceptional circumstances exposure times of 100 ps have been obtained. However, many data sets take seconds to record because of the slow time scale of film change or crystal reorientation or ...
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Entering an era of dynamic structural biology…

BMC Biology, 2018
A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX).
Allen M. Orville
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The RATIO method for time-resolved Laue crystallography [PDF]

Journal of Synchrotron Radiation, 2009
A RATIO method for analysis of intensity changes in time-resolved pump–probe Laue diffraction experiments is described. The method eliminates the need for scaling the data with a wavelength curve representing the spectral distribution of the source and removes the effect of possible anisotropic absorption. It does not require relative scaling of series
Coppens, Philip, Pitak, Mateusz, Gembicky, Milan, Messerschmidt, Marc, Scheins, Stephan, Benedict, Jason, Adachi, Shin-ichi, Sato, Tokushi, Nozawa, Shunsuke, Ichiyanagi, Kohei, Chollet, Matthieu, Shin-ya Koshihara   +11 more
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Time-resolved serial femtosecond crystallography at the European XFEL [PDF]

Nature Methods, 2019
Published by Nature Publishing Group, London [u.a.]
Pandey, Suraj, Bean, Richard, Sato, Tokushi, Poudyal, Ishwor, Bielecki, Johan, Cruz Villarreal, Jorvani, Yefanov, Oleksandr, Mariani, Valerio, White, Thomas A., Kupitz, Christopher, Hunter, Mark, Abdellatif, Mohamed H., Bajt, Saša, Bondar, Valerii, Echelmeier, Austin, Doppler, Diandra, Emons, Moritz, Frank, Matthias, Fromme, Raimund, Gevorkov, Yaroslav, Giovanetti, Gabriele, Jiang, Man, Kim, Daihyun, Kim, Yoonhee, Kirkwood, Henry, Klimovskaia, Anna, Knoska, Juraj, Koua, Faisal H. M., Letrun, Romain, Lisova, Stella, Maia, Luis, Mazalova, Victoria, Meza, Domingo, Michelat, Thomas, Ourmazd, Abbas, Palmer, Guido, Ramilli, Marco, Schubert, Robin, Schwander, Peter, Silenzi, Alessandro, Sztuk-Dambietz, Jolanta, Tolstikova, Alexandra, Chapman, Henry N., Ros, Alexandra, Barty, Anton, Fromme, Petra, Mancuso, Adrian P., Schmidt, Marius   +47 more
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A short history of structure based research on the photocycle of photoactive yellow protein

Structural Dynamics, 2017
The goals of time-resolved macromolecular crystallography are to extract the molecular structures of the reaction intermediates and the reaction dynamics from time-resolved X-ray data alone.
Marius Schmidt
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Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron

IUCrJ, 2020
The recent development of serial crystallography has popularized time-resolved crystallography as a technique to determine the structure of protein-reaction intermediate states.
Sylvain Aumonier, Gianluca Santoni, Guillaume Gotthard, David von Stetten, Gordon A. Leonard, Antoine Royant   +5 more
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A split-beam probe-pump-probe scheme for femtosecond time resolved protein X-ray crystallography

Structural Dynamics, 2015
In order to exploit the femtosecond pulse duration of X-ray Free-Electron Lasers (XFEL) operating in the hard X-ray regime for ultrafast time-resolved protein crystallography experiments, critical parameters that determine the crystallographic signal-to ...
Jasper J. van Thor, Anders Madsen
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Serial X-ray Crystallography

Crystals, 2022
Serial crystallography (SX) is an emerging technique to determine macromolecules at room temperature. SX with a pump–probe experiment provides the time-resolved dynamics of target molecules.
Ki Hyun Nam
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A Perspective on Molecular Structure and Bond-Breaking in Radiation Damage in Serial Femtosecond Crystallography

Crystals, 2020
X-ray free-electron lasers (XFELs) have a unique capability for time-resolved studies of protein dynamics and conformational changes on femto- and pico-second time scales.
Carl Caleman, Francisco Jares Junior, Oscar Grånäs, Andrew V. Martin   +3 more
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