GIGANTEA regulates lateral root formation by modulating auxin signaling in Arabidopsis thaliana
Lateral root (LR) formation is a vital organogenetic process that determines the root architecture in plants. The number of root branches governs the degree of anchorage, efficiency of nutrients acquisition, and water uptake.
Anamika Singh
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Comparing Different Methods of Land use Classification Using the Thermal Band (Case study: Southern Khorasan province) [PDF]
In the present study, four supervised classification algorithms including Maximum Likelihood, Mahalanobis Distance, Minimum Distance and Neural Network with and without TIR1 were used to mapping land use of southern Khorasan province.
Sara Nakhaee Nezhad Fard +4 more
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The role of Phe82 and Phe351 in auxin-induced substrate perception by TIR1 ubiquitin ligase: a novel insight from molecular dynamics simulations. [PDF]
It is well known that Auxin plays a key role in controlling many aspects of plant growth and development. Crystal structures of Transport inhibitor response 1 (TIR1), a true receptor of auxin, were very recently determined for TIR1 alone and in complexes
Ge-Fei Hao, Guang-Fu Yang
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Basic Protocol 1: Generation of eGFP-ARF-P2A-TIR1 or ARF-HA-P2A-TIR1 progenitor cells v1 [PDF]
The first procedure for implementing the ARF-AID system is to establish ARF-TIR1 progenitor cells, as shown in Figure 2A. All the plasmids for integrating ARF-TIR1 into the AAVS1 locus in human cells are available from Addgene. The choice of transfection method varies depending on the cell type; lipofectamine 3000 works efficiently for HEK293T cells.
Kizhakke Mattada Sathyan +2 more
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Gateway vectors for efficient artificial gene assembly in vitro and expression in yeast Saccharomyces cerevisiae [PDF]
Peer reviewedPublisher ...
AL Goldstein +35 more
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Mutant analysis in Arabidopsis provides insight into the molecular mode of action of the auxinic herbicide dicamba. [PDF]
Herbicides that mimic the natural auxin indole-3-acetic acid are widely used in weed control. One common auxin-like herbicide is dicamba, but despite its wide use, plant gene responses to dicamba have never been extensively studied. To further understand
Cynthia Gleason +2 more
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Auxin-independent depletion of degron-tagged proteins by TIR1
The auxin inducible degron (AID) system was developed to enable conditional depletion of proteins of interest. In this system, a plant-derived F-box protein, TIR1, is expressed and complexes with endogenous Skp1 and Cullin proteins to form an E3 ubiquitin ligase complex.
Schiksnis, Erin C +5 more
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The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of
Michael J Prigge +10 more
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Complex regulation of the TIR1/AFB family of auxin receptors [PDF]
Auxin regulates most aspects of plant growth and development. The hormone is perceived by the TIR1/AFB family of F-box proteins acting in concert with the Aux/IAA transcriptional repressors. Arabidopsis plants that lack members of the TIR1/AFB family are auxin resistant and display a variety of growth defects ...
Parry, G. +9 more
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Deeper and longer roots allow crops to survive and flourish, but our understanding of the plant growth regulators promoting root system establishment is limited. Here, we report that, a novel auxin receptor agonist, named K-10, had a remarkable promotive
Zhikun Yang +12 more
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