Results 61 to 70 of about 22,922 (227)

Reconstitution of kinetochore motility and microtubule dynamics reveals a role for a kinesin-8 in establishing end-on attachments

eLife, 2022
During mitosis, individual microtubules make attachments to chromosomes via a specialized protein complex called the kinetochore to faithfully segregate the chromosomes to daughter cells.
Julia R Torvi, Jonathan Wong, Daniel Serwas, Amir Moayed, David G Drubin, Georjana Barnes   +5 more
doaj   +1 more source

TIRF analysis of D. discoideum chemotaxis.

, 2013
A) Bright field and B) TIRF microscopy image sections of cells (8,000 cells mm-2) starved for 5 h on a glass substrate recorded at three distinct time points (I, II, III).
Christian Westendorf (102615), Burkhard Geil (102623), Andreas Janshoff (102626), Edith Schäfer (102606), Noriko Oikawa (102618), Marco Tarantola (102609), Eberhard Bodenschatz (102620), Elena Polo (102612)   +7 more
core   +1 more source

Optical Detection of Cellular Signals at Material Interfaces

Advanced Healthcare Materials, EarlyView.
Emerging functional materials are transforming optical detection of cellular signals. This review highlights optical techniques that exploit the unique optical properties of diverse materials to detect and quantify cellular electrical, chemical, and mechanical signals, and discusses key opportunities and challenges.
Xuchen Ren, Jitong Ren, Zixin Zhang, Koney Eungkon Kim, Francis L. Hong, Yuecheng Peter Zhou   +5 more
wiley   +1 more source

Appropriateness of Prescribing Transmucosal Immediate-Release Fentanyl in the Emergency Room, During Hospitalization, and at Discharge: A Retrospective Study

Pharmaceuticals
Background/Objectives: This study evaluated the appropriateness of transmucosal immediate-release fentanyl (TIRF) prescriptions in a Madrid emergency room during 2019 and 2022, following a 2018 warning about off-label use.
Gary Punjabi, Elena Ramírez
doaj   +1 more source

Optimization of resonant dielectric multilayer for enhanced fluorescence imaging

Optical Materials: X, 2023
In the aim of improving the sensitivity in objective-based total internal reflection fluorescence microscopy (ob-TIRF-M), we recently investigated the use of resonant dielectric multilayer (DM) coated coverslips while considering the experimental ...
A. Mouttou, F. Lemarchand, C. Koc, A. Moreau, J. Lumeau, C. Favard, A.L. Lereu   +6 more
doaj   +1 more source

A transparent waveguide chip for versatile TIRF-based microscopy and nanoscopy

, 2020
Total internal reflection fluorescence microscopy (TIRF) has enabled low-background, live-cell friendly imaging of cell surfaces and other thin samples thanks to the shallow penetration of the evanescent light field into the sample. The implementation of
Nikhil Jayakumar (14380437), Anish Priyadarshi (9917424), Azeem Ahmad (830230), Jean-Claude Tinguely (4286566), Vishesh Dubey (14406417), Ganapathy Senthil Murugan (3959378), Firehun Tsige Dullo (17512650), Deanna L. Wolfson (17516178), Balpreet Singh Ahluwalia (3978188)   +8 more
core   +1 more source

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

Alternative Methods to Enhance the Axial Resolution of Total Internal Reflection Fluorescence–Structured Illumination Microscopy

Photonics
Total internal reflection fluorescence–structured illumination microscopy (TIRF-SIM) can enhance the lateral resolution of fluorescence microscopy to twice the diffraction limit, enabling subtler observations of activity in subcellular life. However, the
Xiu Zheng, Xiaomian Cai, Wenjie Liu, Youhua Chen, Cuifang Kuang   +4 more
doaj   +1 more source

Unusually strong temperature dependence of P2X3 receptor traffic to the plasma membrane

Frontiers in Cellular Neuroscience, 2011
ATP-gated P2X3 receptors are expressed by nociceptive neurons and participate in transduction of pain. Responsiveness of P2X3 receptors is strongly enhanced at high temperatures, suggesting a role for these receptors in temperature detection.
Evgeny ePryazhnikov, Dmitriy eFayuk, Minna eNiittykoski, Rashid eGiniatullin, Leonard eKhiroug   +4 more
doaj   +1 more source

Chromatin Packing Domain Engineering Through the Manipulation of Nuclear Cationic States

Advanced Science, EarlyView.
Manipulation of intranuclear divalent cation concentrations rapidly and reversibly remodels chromatin packing domains in living cells. Magnesium depletion disrupts domain compaction, heterochromatin organization, and transcriptional regulation, whereas magnesium loading promotes more compact and stable domains.
Cody L. Dunton, Carrillo Paola Gonzalez, Luay Almassalha, Wing Shun Li, Ruyi Gong, Nicolas Acosta, I Chae Ye, Jane Frederick, Christopher Hogg, Saira John, J. A. Gareth Williams, Rikkert J. Nap, Igal Szleifer, Vadim Backman   +13 more
wiley   +1 more source