Results 51 to 60 of about 22,922 (227)
near-TIRF sqhGFP fosmid and cell mask far-red
, 2021 movies taken in near TIRF, 20 sec per frame, of stage 7 egg chambers expressing myosin regulatory light chain tagged with GFP (Vienna Drosophila Resource center fosmid line 314484) and stained with far-red cell Mask.
Kristin Sherrard (11662114)
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Image analysis optimization for nanowire-based optical detection of molecules
NanophotonicsSemiconductor nanowires can enhance the signal of fluorescent molecules, thus significantly improving the limits of fluorescence detection in optical biosensing.
Davtyan Rubina +4 more
doaj +1 more source
Bio-Protocol, 2017 Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing the dynamics of actin filaments at single-filament resolution in vitro.
Yuxiang Jiang, Shanjin Huang
doaj +1 more source
Redefining the specificity of phosphoinositide-binding by human PH domain-containing proteins
Nature Communications, 2021 Pleckstrin homology (PH) domains are presumed to bind phosphoinositides (PIPs) but only few specific interactions are known. Using TIRF microscopy based assays, functional studies and an experimentally validated prediction algorithm, the authors show ...
Nilmani Singh +6 more
doaj +1 more source
Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy [PDF]
Journal of Visualized Experiments, 2009 Total internal reflectance fluorescence (TIRF) microscopy is a technique that allows the study of events happening at the cell membrane, by selective imaging of fluorescent molecules that are closest to a high refractive index substance such as glass. In this article, we apply this technique to image exocytosis of synaptic vesicles in retinal bipolar ...
Joselevitch, Christina, Zenisek, David
openaire +2 more sources
Identifying rhodopsin-containing cells using TIRF microscopy
, 2015 Accepted manuscript posted online.Sunlight is captured and converted to chemical energy in illuminated environments. Although (bacterio)chlorophyll-based photosystems have been characterized in detail, retinal-based photosystems, rhodopsins, have only ...
Maresca, Julia Anne +5 more
core +1 more source
How is Nanoarchitectonics Shaping Extracellular Vesicle Research?
Advanced Healthcare Materials, EarlyView.The convergence of nanoarchitectonics and extracellular vesicle biology is reshaping next‐generation nanomedicine by enabling rationally designed nanostructures for enhanced EVs detection, engineering, and therapy. ABSTRACT Extracellular vesicles (EVs) have gained recognition as crucial mediators of cell‐to‐cell communication, holding immense potential
Madushani Dahanayake +5 more
wiley +1 more source
Journal of Imaging, 2019 Total internal reflection fluorescence microscopy with polarized excitation (P-TIRF) can be used to image nanoscale curvature phenomena in live cells. We used P-TIRF to visualize rat basophilic leukemia cells (RBL-2H3 cells) primed with fluorescent anti ...
Rosa Machado +4 more
doaj +1 more source
Nature Communications, 2021 Uniform illumination is a prerequisite for quantitative analyses in both classical fluorescence microscopy and single molecule localisation microscopy. Here, the authors introduce ASTER, an illumination technique that generates uniform illumination over ...
Adrien Mau +4 more
doaj +1 more source