Results 31 to 40 of about 22,922 (227)
Molecular Basis for Actin Polymerization Kinetics Modulated by Solution Crowding
Biomolecules, 2023 Actin polymerization drives cell movement and provides cells with structural integrity. Intracellular environments contain high concentrations of solutes, including organic compounds, macromolecules, and proteins.
Bryan Demosthene +4 more
doaj +1 more source
Dynamics of cell membrane lesions and adaptive conductance under the electrical stress
Cell StressExceeding physiological limits of the cell membrane potential compromises structural integrity, enabling the passage of normally impermeant solutes and disrupting cell function.
Mantas Silkunas +3 more
doaj +1 more source
Communications Biology, 2021 With fluorescence based-TIRF microspectroscopy, Ušaj et al. unveil mechanistic details about the ATP turnover rates by myosin and actomyosin with enzymatic reaction pathways that were not possible to obtain from ensemble studies.
Marko Ušaj +4 more
doaj +1 more source
Imaging Microtubules in vitro at High Resolution while Preserving their Structure
Bio-Protocol, 2021 Microtubules (MT) are the most rigid component of the cytoskeleton. Nevertheless, they often appear highly curved in the cellular context and the mechanisms governing their overall shape are poorly understood.
Camille Cuveillier +3 more
doaj +1 more source
Improved field uniformity with shadowless TIRF excitation.
, 2015 (A, B) Images (500x300 pixel, 40 x 24 μm) of rhodamine film on a coverglass. The image in A was captured using stationary-spot (conventional) TIRF excitation at 488 nm; that in B shows the same specimen but imaged by spinning-spot TIRF excitation.
Ian Parker (101) +2 more
core +1 more source
Optimization of an optical setup for combined TIRF/confocal fluorescence microscopy [PDF]
, 2021 This work deals with the construction of a microscope for combined total internal reflection fluorescence (TIRF) and confocal microscopy. It is especially designed for single-molecule fluorescence spectroscopy.
Börner, Richard, Michaelsen, Hannes
core +1 more source
TIRF imaging data of neutrophils migrating underneath endothelial cells
, 2021 This data is used in the publication "Endothelial Focal Adhesions Are Functional Obstacles for Leukocytes During Basolateral Crawling": https://www.frontiersin.org/articles/10.3389/fimmu.2021.667213/full TIRF Microscopy Lentiviral transduction was ...
Goedhart, Joachim, Mahlandt, Eike
core +2 more sources
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
Nature Communications, 2019 The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by ...
Florian Stehr +4 more
doaj +1 more source
Total Internal Reflection Fluorescence (TIRF) Microscopy [PDF]
Current Protocols in Cytometry, 2009 AbstractTotal internal reflection fluorescence (TIRF) microscopy (TIRFM) is an elegant optical technique that provides for the excitation of fluorophores in an extremely thin axial region (“optical section”). The method is based on the principle that when excitation light is totally internally reflected in a transparent solid (e.g., coverglass) at its ...
openaire +3 more sources
Minimal design inverted TIRF microscope.
, 2013 a) A photograph of the instrument, the view on the objective is hidden by the x-y sample stage. The inset cartoon shows the open-top sample chamber. b) A cross-sectional drawing of the essential components of the instrument. The TIRF objective is mounted
Max Linke (494931) +4 more
core +1 more source