A framework for multi-angle TIRF microscope calibration [PDF]
2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI), 2016 This communication presents a pipeline for Multi-Angle TIRF calibration from the measurement of the incident angle to the model validation. This problem is of major importance when dealing with 3D reconstruction methods from a set of MA-TIRF acquisitions since the reconstruction accuracy highly depends on the agreement between the theoretical model and
Soubies, Emmanuel +5 more
openaire +4 more sources
Biomedical Optics Express, 2014 Total-internal-reflection fluorescence (TIRF) microscopy provides high optical-sectioning capability and a good signal-contrast ratio for structures near the surfaces of cells. In recent years, several improvements have been developed, such as variable-angle TIRF (VA-TIRF) and spinning TIRF (sp-TIRF), which permit quantitative image analysis and ...
Weijian, Zong +6 more
openaire +3 more sources
Immersed-Prism TIRF Microscopy for Visualizing Intraflagellar Transport in Live Cells
PhotonicsTotal internal reflection fluorescence (TIRF) microscopy excites fluorophores within a few hundred nanometers of the sample–substrate interface, enabling high-contrast imaging near the cell membrane.
Maya Chauhan +4 more
doaj +2 more sources
3D Image super-resolution by fluorophore fluctuations and MA-TIRF microscopy reconstruction (3D-COL0RME) [PDF]
, 2022 International audienceWe propose a 3D super-resolution approach to improve both lateral and axial spatial resolution in Total Internal Reflectance Fluorescence (TIRF) imaging applications.
Blanc-Féraud, Laure +3 more
core +8 more sources
Force determination in lateral magnetic tweezers combined with TIRF microscopy [PDF]
Nanoscale, 2018 We have designed and calibrated a magnetic tweezers module to laterally stretch DNA molecules at a constant force, which can be incorporated into conventional magnetic tweezers. We demonstrate the combination of lateral magnetic tweezers with TIRF microscopy by characterizing DNA binding by ParB.
J. Madariaga-Marcos +5 more
openaire +7 more sources
Paclitaxel induces NM2-dependent cellular contraction through GEF-H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells. [PDF]
Mol OncolTaxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Asensio-Juárez G +5 more
europepmc +2 more sources
Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella [PDF]
, 2009 The eukaryotic flagellum is host to a variety of dynamic behaviors, including flagellar beating, the motility of glycoproteins in the flagellar membrane, and intraflagellar transport (IFT), the bidirectional traffic of protein particles between the ...
Wallace F. Marshall +11 more
core +2 more sources
Integrating Lateral Super-Resolution and Axial Progression Reveals Distinct Clathrin Pit Formation Pathways. [PDF]
TrafficVariable‐angle total internal reflection fluorescence structured illumination microscopy (vaTIRF‐SIM) links nanoscale lateral organization with axial progression during clathrin‐mediated endocytosis. Super‐resolved TIRF‐SIM images resolve the lateral architecture of AP2‐labeled clathrin coats, while corresponding Z‐index maps report relative axial ...
Thompson C +3 more
europepmc +2 more sources
The role of clathrin in post-Golgi secretion in plant cells. [PDF]
New PhytolMorphology of Arabidopsis thaliana XVE»amiCHC seedlings. Summary Within the plant endomembrane system, the vesicle coat protein clathrin localizes to the plasma membrane (PM) and the trans‐Golgi Network/early endosome (TGN/EE). While the role of clathrin in endocytosis at the PM is well established, its function at TGN/EE, presumably in late secretion (
Adamowski M +4 more
europepmc +2 more sources
Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS). [PDF]
, 2012 We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion (STED) nanoscopy.
Eggeling, C. +14 more
core +2 more sources