Results 41 to 50 of about 22,922 (227)
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
PLoS ONE, 2011 Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle ...
Edgar Gutierrez +7 more
doaj +1 more source
Analysis of spatial distribution of KIR2DL2 receptors extracted from TIRF imaging data.
, 2022 (A) Shows initial configuration of KIR2DL2 at t = 0 in our simulations based on the coarse-grained 2D image extracted for a region of interest in TIRF image (Fig 4 in Ref.
Rajdeep Kaur Grewal (12563150) +1 more
core +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Mechanochemical tuning of a kinesin motor essential for malaria parasite transmission
Nature Communications, 2022 Plasmodium kinesin-8B is essential for male gamete formation and its absence blocks parasite transmission. Using cryo-EM and TIRF, the authors report how kinesin-8B motor domains are tuned to support microtubule motility and depolymerase activity.
Tianyang Liu +8 more
doaj +1 more source
TIRF microscopy with ultra-short penetration depth
Optics Express, 2014 Total internal reflection fluorescence microscopy (TIRF), in both commercial and custom-built configurations, is widely used for high signal-noise ratio imaging. The imaging depth of traditional TIRF is sensitive to the incident angle of the laser, and normally limited to around 100 nm.
Hao, Shen +5 more
openaire +2 more sources
Correlation between TIRF and ECIS experiment.
, 2013 A) Oscillating gray tone of subtracted bright field images (red) in comparison to the total fluorescence intensity of the corresponding TIRF images (green) and the impedance signal (black) as a function of time.
Christian Westendorf (102615) +7 more
core +1 more source
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
A detection-based framework for the analysis of recycling in TIRF microscopy [PDF]
2010 IEEE International Symposium on Biomedical Imaging: From Nano to Macro, 2010 Endocytosis/recycling and exocytosis are mechanisms conserved through evolution allowing cells to communicate with their external medium. In order to study these dynamic processes, the present work proposes a patch-based method for detecting recycling or exocytotic events at the Plasma membrane in fast TIRF microscopy combined with the computation of ...
Chessel, Anatole +5 more
openaire +2 more sources
Schematic of the optical design for TIRF illumination.
, 2012 Schematic of the optical design for TIRF illumination.
Karoline Wiesner (188408) +3 more
core +1 more source