Results 21 to 30 of about 22,922 (227)

Characterizing the bio-functionalization of gold surface with total internal reflection fluorescence (TIRF) microscopy [PDF]

Proceedings of the Estonian Academy of Sciences, 2020
Quality of bioactive surface is crucial for achieving the required sensitivity and selectivity of biosensing systems. Numerous methods are available for the characterization of metal-coated surfaces, but only a few to test the efficacy of biomaterial ...
Robin Ehrminger, Sergei Kopanchuk, Kairi Kivirand, Tavo Romann, Toonika Rinken, Mart Min, Ago Rinken   +6 more
doaj   +1 more source

Quantifying exocytosis by combination of membrane capacitance measurements and total internal reflection fluorescence microscopy in chromaffin cells.

PLoS ONE, 2007
Total internal reflection fluorescence microscopy (TIRF-Microscopy) allows the observation of individual secretory vesicles in real-time during exocytosis.
Ute Becherer, Mathias Pasche, Shahira Nofal, Detlef Hof, Ulf Matti, Jens Rettig   +5 more
doaj   +1 more source

Total Internal Reflection Fluorescence Quantification of Receptor Pharmacology

Biosensors, 2015
Total internal reflection fluorescence (TIRF) microscopy has been widely used as a single molecule imaging technique to study various fundamental aspects of cell biology, owing to its ability to selectively excite a very thin fluorescent volume ...
Ye Fang
doaj   +1 more source

Study of Applicability of Triangular Impulse Response Function for Ultimate Strength of LNG Cargo Containment Systems under Sloshing Impact Loads

Applied Sciences, 2023
The LNG cargo containment system used in membrane-type LNG cargo tanks must have sufficient dynamic strength to withstand the impact of sloshing loads.
Young IL Park, Seung Ha Lee, Jeong-Hwan Kim   +2 more
doaj   +1 more source

Functional significance of HCM mutants of tropomyosin, V95A and D175N, studied with in vitro motility assays

Biophysics and Physicobiology, 2019
The majority of hypertrophic cardiomyopathy (HCM) is caused by mutations in sarcomere proteins. We examined tropomyosin (Tpm)’s HCM mutants in humans, V95A and D175N, with in vitro motility assay using optical tweezers to evaluate the effects of the Tpm ...
Shuya Ishii, Madoka Suzuki, Shin’ichi Ishiwata, Masataka Kawai   +3 more
doaj   +1 more source

A Platform for Combined DNA and Protein Microarrays Based on Total Internal Reflection Fluorescence

Sensors, 2012
We have developed a novel microarray technology based on total internal reflection fluorescence (TIRF) in combination with DNA and protein bioassays immobilized at the TIRF surface.
Alexander Asanov, Angélica Zepeda, Luis Vaca   +2 more
doaj   +1 more source

A1907 raw TIRF data

, 2021
Raw data from TIRF microscope. The detailed description is in the full manuscript.
Cifra, Michal, Havelka, Daniel, Zhernov, Ilia   +2 more
openaire   +2 more sources

easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy [PDF]

, 2016
TIRF and STORM microscopy are super-resolving fluorescence imaging modalities for which current implementations on standard microscopes can present significant complexity and cost. We present a straightforward and low-cost approach to implement STORM and
Mark A.A. Neil, Timothy Davies, Marco A. Purbhoo, Ian Munro, Purbhoo, MA, Davies, T, Savell, A, Munro, I, Kwasi Kwakwa, Alexander Savell, Kwakwa, K, French, PMW, Parrinello, S, Neil, MAA, Dunsby, C, Simona Parrinello, Chris Dunsby, Paul M.W. French   +17 more
core   +1 more source

E-scooter riders: A cross-cultural analysis of traffic safety attitudes and behaviors

IATSS Research
The rapid adoption of electric scooters (e-scooters) has transformed urban mobility, offering a practical and flexible alternative to traditional transportation modes, particularly in areas with limited access to public transit.
Milad Delavary, Craig Lyon, Ward G.M. Vanlaar, Robyn D. Robertson, Dimitrios Nikolaou, George Yannis   +5 more
doaj   +1 more source

Steady-state acceptor fluorescence anisotropy imaging under evanescent excitation for visualisation of FRET at the plasma membrane.

PLoS ONE, 2014
We present a novel imaging system combining total internal reflection fluorescence (TIRF) microscopy with measurement of steady-state acceptor fluorescence anisotropy in order to perform live cell Förster Resonance Energy Transfer (FRET) imaging at the ...
Viviane Devauges, Daniel R Matthews, Justin Aluko, Jakub Nedbal, James A Levitt, Simon P Poland, Oana Coban, Gregory Weitsman, James Monypenny, Tony Ng, Simon M Ameer-Beg   +10 more
doaj   +1 more source