Results 1 to 10 of about 17,264 (162)
Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM) [PDF]
Nature Communications, 2021 Quantifying rapid and small cellular forces is a major challenge in mechanobiology. Here, the authors show a >2-fold spatially and >10-fold temporally force sampling improvement combining traction force microscopy with total internal reflection ...
Liliana Barbieri +12 more
doaj +4 more sources
Spinning-Spot Shadowless TIRF Microscopy. [PDF]
PLoS ONE, 2015 Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing near-membrane cellular structures and processes, including imaging of local Ca2+ transients with single-channel resolution.
Kyle L Ellefsen +2 more
doaj +6 more sources
Setting up multicolour TIRF microscopy down to the single molecule level
Biomolecular Concepts, 2023 Investigating biological mechanisms in ever greater detail requires continuous advances in microscopy techniques and setups. Total internal reflection fluorescence (TIRF) microscopy is a well-established technique for visualizing processes on the cell ...
Chiara Schirripa Spagnolo, Stefano Luin
exaly +2 more sources
New light on ion channel imaging by total internal reflection fluorescence (TIRF) microscopy
Journal of Pharmacological Sciences, 2015 Ion channels play pivotal roles in a wide variety of cellular functions; therefore, their physiological characteristics, pharmacological responses, and molecular structures have been extensively investigated.
Hisao Yamamura +2 more
exaly +3 more sources
HardwareX, 2023 Imaging the dynamics and interactions of biomolecules at the single-molecule level in live cells and reconstituted systems has generated unprecedented knowledge about the biomolecular processes underlying many cellular functions. To achieve the speed and
Kristina A Ganzinger +1 more
exaly +3 more sources
Prevalence and Patterns of Off-Label Transmucosal Immediate-Release Fentanyl Use in French General Practice: A Mixed-Methods Study Based on a Voluntary Online Survey. [PDF]
Pain TherIntroduction Prescriptions of transmucosal immediate-release fentanyl (TIRF) have increased in recent years, driven by their rapid onset and ease of administration compared to other opioids like morphine and oxycodone, especially for breakthrough pain. A
Guastella V +5 more
europepmc +2 more sources
Colour-coded nanoscale calibration and optical quantification of axial fluorophore position [PDF]
EPJ Web of Conferences, 2023 Total internal reflection fluorescence (TIRF) has come of age, but a reliable and easy-to-use tool for calibrating evanescent-wave penetration depths is missing.
Olevsko-Arad Ilya +3 more
doaj +1 more source
STAR Protocols, 2021 Summary: This protocol outlines steps to visualize and detect Ca2+ puffs following photo-liberation of caged inositol-1,4,5-trisphosphate (IP3) from HEK-293 cells expressing only the native IP3R type 1 receptor using total internal reflection ...
Vikas Arige +4 more
doaj +1 more source
TIRFing out Studies on Glut4 Trafficking [PDF]
Developmental Cell, 2007 Docking and fusion of Glut4 vesicles with the plasma membrane are essential but poorly understood steps during insulin-stimulated glucose transport. Recent studies utilizing TIRF microscopy shed light on these processes and map the sites of possible intervention by insulin from just underneath the plasma membrane.
Chen, Xiao-Wei, Saltiel, Alan R.
openaire +2 more sources
Biology, 2021 Single-molecule total internal reflection fluorescence (TIRF) microscopy allows for the real-time visualization of macromolecular dynamics and complex assembly.
Max S. Fairlamb +4 more
doaj +1 more source