Results 71 to 80 of about 22,922 (227)
Data, 2019 Total internal reflection fluorescence (TIRF) microscope image sequences are commonly used to study receptors in live cells. The dataset presented herein facilitates the study of the IgE-FcεRI receptor signaling complex (IgE-RC) in rat basophilic ...
Rachel Drawbond, Kathrin Spendier
doaj +1 more source
Photonic-chip assisted correlative light and electron microscopy
Communications Biology, 2020 Tinguely et al. develop a photonic chip-based correlative light-electron microscopy system to generate co-registered multi-modal total internal reflection fluorescence microscopy (TIRF) and electron microscopy (EM) images of biological samples at ...
Jean-Claude Tinguely +8 more
doaj +1 more source
Single-Molecule Studies of Membrane Receptors from Brain Region Specific Nanovesicles
Bio-Protocol, 2021 Single molecule imaging and spectroscopy are powerful techniques for the study of a wide range of biological processes including protein assembly and trafficking.
Surya Aryal +4 more
doaj +1 more source
Advanced Science, EarlyView.A mechanically activated SynNotch reporter platform enables detection of force transmission through receptors as they mediate immune cell interactions. Targeting CD40 on B cells and TCR on T cells records force‐induced reporter activation across 2D coculture, 3D organoid, and implanted in mice, which also reveal force‐amplification of immune receptor ...
Menglan Li +9 more
wiley +1 more source
Scientific Reports, 2021 Clostridium botulinum neurotoxin serotype A (BoNT/A) is a potent neurotoxin that serves as an effective therapeutic for several neuromuscular disorders via induction of temporary muscular paralysis.
Nicholas G. James +7 more
doaj +1 more source
Advanced Science, EarlyView.The protein aggregates and gene expression in the middle temporal gyrus (MTG) and somatosensory cortex (SOM) of the postmortem brains of 13 Alzheimer's disease patients were studied in detail, revealing that small hyperphosphorylated tau aggregates increase with Braak stage driven by microglial inflammation.
Elizabeth A. English +9 more
wiley +1 more source
Advanced Science, EarlyView.Tau populations with different aggregation states associate with distinct sets of proteins in the Alzheimer's disease brain. Proteomic discovery combined with single‐molecule analysis in neurons reveals dynamic associations involving proteostasis, metabolism and RNA biology.
Dorothea Böken +8 more
wiley +1 more source
Advanced Energy Materials, EarlyView.This review looks at the different experimental techniques that measure spatiotemporal charge carrier dynamics. This information is viewed in the context of particulate photocatalysts, outlining the insights these techniques provide and how they advance our understanding.
Sutripto Khasnabis, Robert Godin
wiley +1 more source
Near-wall dynamics of single cavitation bubbles imaged by total internal reflection fluorescence
Ultrasonics SonochemistryThis study introduces total internal reflection fluorescence (TIRF) as a method for investigating the dynamics of a single laser-induced cavitation bubble in water within a thin layer near a solid surface.
Fangyi Wang +2 more
doaj +1 more source
Calibrating Evanescent-Wave Penetration Depths for Biological TIRF Microscopy [PDF]
Biophysical Journal, 2019 Roughly half of a cells proteins are located at or near the plasma membrane. In this restricted space the cell senses its environment, signals to its neighbors and ex-changes cargo through exo- and endocytotic mechanisms. Ligands bind to receptors, ions flow across channel pores, and transmitters and metabolites are transported against con-centration ...
Oheim, Martin +4 more
openaire +3 more sources