Aspergillus fumigatus enhances elastase production in pseudomonas aeruginosaco-cultures [PDF]
, 2015 In the cystic fibrosis (CF) lung the presence of bacteria and fungi in the airways promotes an inflammatory response causing progressive lung damage, ultimately leading to high rates of morbidity and mortality.
Kerr, Stephen +6 more
core +1 more source
Mapping the evolution of mitochondrial complex I through structural variation
FEBS Letters, EarlyView.Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin +2 more
wiley +1 more source
Iyer Laboratory Solid Tissue Chromatin Immunoprecipitation Protocol [PDF]
, 2017 A detailed protocol on how to perform chromatin immunoprecipitation in solid tissue samples.
Hall, Amelia
core +1 more source
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
FEBS Letters, EarlyView.This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Seeing through Musculoskeletal Tissues: Improving In Situ Imaging of Bone and the Lacunar Canalicular System through Optical Clearing. [PDF]
PLoS ONE, 2016 In situ, cells of the musculoskeletal system reside within complex and often interconnected 3-D environments. Key to better understanding how 3-D tissue and cellular environments regulate musculoskeletal physiology, homeostasis, and health is the use of ...
Ian M Berke +4 more
doaj +1 more source
Clearing-induced tisssue shrinkage: A novel observation of a thickness size effect.
PLoS ONE, 2021 The use of clearing agents has provided new insights in various fields of medical research (developmental biology, neurology) by enabling examination of tissue architecture in 3D.
R C M Vulders +3 more
doaj +1 more source
Tissue Processor Based PLC (Programmable Logic Controller) [PDF]
, 2019 Tissue Processor Tissue Processor consists of consists of several stages of dehydration, clearing, and paraffin infiltration.Phase dehydration to remove water content in tissues by immersion into alcohol.
Ariswati, H. G. (Her) +2 more
core
Effects of endocrine-disrupting chemical exposure on zebrafish ovarian follicles [PDF]
, 2017 The process of oocyte maturation involves both nuclear and cytoplasmic events that are essential for the production of viable eggs and subsequent fertilization in sexually reproducing animals.
D\u27Angelo, Josephine +1 more
core +2 more sources
Optimizing probes to image cleared tissue [PDF]
Nature Methods, 2016 observation that led to Scale, his urea-based clearing agent. It removes lipids with detergents rather than with organic solvents and then hydrates tissue. A new sorbitol and urea–based version called ScaleS is gentler, says Miyawaki, and it preserves membrane structures well. Thus, for example, fluorescent proteins tethered to the plasma membrane will
openaire +2 more sources
By dawn or dusk—how circadian timing rewrites bacterial infection outcomes
FEBS Letters, EarlyView.The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo +2 more
wiley +1 more source