Results 51 to 60 of about 2,240,094 (340)

In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]

, 2010
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada, Barbero, Andrea, Candrian, Christian, Gianni-Barrera, Roberto, Jaquiery, Claude, Juelke, Henriette, Mainil-Varlet, Pierre, Martin, Ivan, Miot, Sylvie, Pelttari, Karoliina   +9 more
core   +1 more source

Single ChAdOx1 nCoV‐19 dose elicits stronger immune response in previously infected individuals than in SARS‐CoV2 naive persons

Immunity, Inflammation and Disease
Background Current vaccines against COVID‐19 effectively reduce morbidity and mortality and are vitally important for controlling the pandemic. Between December 2020 and February 2021, adenoviral vector vaccines such as ChAdOx1 (AstraZeneca‐Oxford) were ...
Fawzi Ebrahim, Asma Alboueishi, Inas M. Alhudiri, Salah Al Tabal, Yosra Lamami, Samira Al Dwigen, Sondos Ashleb, Noha Ejenfawi, Mohamed B. Milad, Hayat Rhoumah, Salah Edin El Meshri, Adam Elzagheid   +11 more
doaj   +1 more source

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

CULTURES OF ORGANIZED TISSUES [PDF]

Journal of Experimental Medicine, 1922
An artificial organism, if one may so term it, composed of a complex of tissues, was cultivated for a long period of time. Small fragments of intestine from chick embryos 20 to 21 days old were placed in a suitable medium. The epithelium proliferated and completely covered the fragment of intestine after 4 to 6 days. A small body was thus formed, round
openaire   +3 more sources

The nicotinamide hypothesis revisited—plant defense signaling integrating PARP, nicotinamide, nicotinic acid, epigenetics, and glutathione

FEBS Letters, EarlyView.
Nicotinamide (NIC) and nicotinic acid (NIA) are proposed as stress signaling compounds in plants. Oxidative stress may lead to single strand breaks (SSB) in DNA, which activate poly(ADP‐ribose) polymerase (PARP). NIC and NIA are then formed from NAD. NIC and NIA can promote epigenetic changes leading to the expression of defense genes specific for the ...
Torkel Berglund, Anna B. Ohlsson
wiley   +1 more source

Elevational adaptation and phenotypic plasticity of Codonopsis clematidea in the trans-Himalaya

Discover Plants
The process of adapting to changing environmental conditions plays a pivotal role in driving plant diversification. Elevational gradients provide a unique opportunity to investigate adaptation to diverse climatic conditions.
Zarina Khatoon, Seema Singh, Kanize Fatima, Mohd Ibrahim, Sajjad Ali, Sharada Mallubhotla   +5 more
doaj   +1 more source

C9orf72 ALS‐causing mutations lead to mislocalization and aggregation of nucleoporin Nup107 into stress granules

FEBS Letters, EarlyView.
Mutations in the C9orf72 gene represent the most common genetic cause of amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disease. Using patient‐derived neurons and C. elegans models, we find that the nucleoporin Nup107 is dysregulated in C9orf72‐associated ALS. Conversely, reducing Nup107 levels mitigates disease‐related changes.
Saygın Bilican, Yara Nabawi, William Hongyu Zhang, Dunja Petrovic, Markus Wehrmann, Sara Muñoz‐García, Seda Koyuncu, David Vilchez   +7 more
wiley   +1 more source

Imeglimin attenuates liver fibrosis by inhibiting vesicular ATP release from hepatic stellate cells

FEBS Letters, EarlyView.
Imeglimin, at clinically relevant concentrations, inhibits vesicular ATP accumulation and release from hepatic stellate cells, thereby attenuating purinergic signaling and reducing fibrogenic activation. This mechanism reveals a newly identified antifibrotic action of imeglimin beyond glycemic control.
Seiji Nomura, Lixiang Wang, Nao Hasuzawa, Ayako Nagayama, Sawako Moriyama, Kenji Ashida, Yoshinori Moriyama, Masatoshi Nomura, Ken Yamamoto   +8 more
wiley   +1 more source

Tissue Microenvironments Define and Get Reinforced by Macrophage Phenotypes in Homeostasis or during Inflammation, Repair and Fibrosis [PDF]

, 2012
Current macrophage phenotype classifications are based on distinct in vitro culture conditions that do not adequately mirror complex tissue environments.
Weidenbusch, Marc, Anders, Hans-Joachim
core   +1 more source

Uncovering mercury accumulation and the potential for bacterial bioremediation in response to contamination in the Singalila National Park

Scientific Reports
Several recent investigations into montane regions have reported on excess mercury accumulation in high-altitude forest ecosystems. This study explored the Singalila National Park, located on the Singalila ridge of the Eastern Himalayas, revealing ...
Sukanya Acharyya, Soumya Majumder, Sudeshna Nandi, Arindam Ghosh, Sumedha Saha, Malay Bhattacharya   +5 more
doaj   +1 more source