Results 161 to 170 of about 968,773 (372)
FEBS Open Bio, Volume 15, Issue 6, Page 898-905, June 2025.We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei +6 more
wiley +1 more source
Rapid prediction of organisation in engineered corneal, glial and fibroblast tissues using machine learning and biophysical models [PDF]
arXivWe present a machine learning approach for predicting the organisation of corneal, glial and fibroblast cells in 3D cultures used for tissue engineering. Our machine-learning-based method uses a powerful generative adversarial network architecture called pix2pix, which we train using results from biophysical contractile network dipole orientation ...
arxiv
FEBS Open Bio, Volume 15, Issue 6, Page 914-921, June 2025.Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley +1 more source
Comparison of chronic wound culture techniques: swab versus curetted tissue for microbial recovery [PDF]
, 2014 Maria Elisa Smith +3 more
openalex +1 more source
Cartilage Tissue Engineering: the effect of different biomaterials, cell types and culture methods [PDF]
, 2006 Chapter 1 outlines the normal structure and composition of articular cartilage and the inefficient spontaneous healing response after focal damage.
Marijnissen, W.J.C.M. (Willem)
core +2 more sources
FEBS Open Bio, EarlyView.The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik +3 more
wiley +1 more source
Assessment of pancreas cells [PDF]
Pancreatic islets were obtained from guinea pig pancreas by the collagenase method and kept alive in tissue culture prior to further studies. Pancreas cell morphology was studied by standard histochemical techniques using light microscopy.
Vanoss, C. J.
core +1 more source
FEBS Open Bio, Volume 15, Issue 6, Page 994-1008, June 2025.SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel +13 more
wiley +1 more source
Regulation of endothelial cell arrangements within hMSC – HUVEC co-cultured aggregates
Biomedical Journal, 2019 Background: Micro-mass culturing or cellular aggregation is an effective method used to form mineralised bone tissue. Poor core cell viability, however, is often an impeding characteristic of large micro-mass cultures, and equally for large tissue ...
Anthony J. Deegan +4 more
doaj
MyData: A Comprehensive Database of Mycetoma Tissue Microscopic Images for Histopathological Analysis [PDF]
arXivMycetoma is a chronic and neglected inflammatory disease prevalent in tropical and subtropical regions. It can lead to severe disability and social stigma. The disease is classified into two types based on the causative microorganisms: eumycetoma (fungal) and actinomycetoma (bacterial).
arxiv