Results 61 to 70 of about 621,620 (285)

Non-specific binding of antibodies in immunohistochemistry: Fakes and facts [PDF]

, 2011
Protocols for blocking non-specific antibody (Ab) binding in immunohistochemistry are based on rather contradictory and outdated reports. This prompted us to prove, whether non-specific Ab binding may really lead to unwanted background staining in ...
Igor Buchwalow, Markus Tiemann, Vera Samoilova, Werner Boecker   +3 more
core   +1 more source

Reduced vascular leakage correlates with breast carcinoma T regulatory cell infiltration but not with metastatic propensity

Molecular Oncology, EarlyView.
A mouse model for vascular normalization and a human breast cancer cohort were studied to understand the relationship between vascular leakage and tumor immune suppression. For this, endothelial and immune cell RNAseq, staining for vascular function, and immune cell profiling were employed.
Liqun He, Chiara Testini, Neda Hekmati, Altea Bonello, Aglaia Schiza, Emmanuel Nwadozi, Mia Phillipson, Carina Strell, Michael Welsh   +8 more
wiley   +1 more source

Quality assessment of histopathological stainings on prolonged formalin fixed thrombus tissues retrieved by mechanical thrombectomy

Frontiers in Neurology, 2023
BackgroundFormalin-fixed retrieved clots from mechanical thrombectomy (MT) are now routinely studied using both conventional histopathologic techniques and immunohistochemistry (IHC).
Cem Bilgin, Daying Dai, Collin Johnson, Oana M. Mereuta, David F. Kallmes, Waleed Brinjikji, Ramanathan Kadirvel, Ramanathan Kadirvel   +7 more
doaj   +1 more source

Monoclonal antibodies against human astrocytomas and their reactivity pattern [PDF]

, 1987
The establishment of hybridomas after fusion of X63-Ag8.653 mouse myeloma cells and splenocytes from mice hyperimmunized against human astrocytomas is presented. The animals were primed with 5 × 106 chemically modified uncultured or cultured glioma cells.
B. Martin, Bourdon, Bullard, Bullard, Carrel, Coakham, Coakham, D. Stavrou, De Muralt, E. Keiditsch, Epenetos, F. Schmidberger, I. Funke, Jonak, K. Bise, Kearney, Kemshead, Krajewski, Köhler, Lanier, Posner, R. Kurrle, Roholl, Schnegg, Seeger, Sikora, Stavrou, Stavrou, Stavrou, Stavrou, Stavrou, Stavrou, Stavrou, Taub, U. Stocker, W. Eisenmenger, Wikstrand   +36 more
core   +1 more source

Class IIa HDACs forced degradation allows resensitization of oxaliplatin‐resistant FBXW7‐mutated colorectal cancer

Molecular Oncology, EarlyView.
HDAC4 is degraded by the E3 ligase FBXW7. In colorectal cancer, FBXW7 mutations prevent HDAC4 degradation, leading to oxaliplatin resistance. Forced degradation of HDAC4 using a PROTAC compound restores drug sensitivity by resetting the super‐enhancer landscape, reprogramming the epigenetic state of FBXW7‐mutated cells to resemble oxaliplatin ...
Vanessa Tolotto, Nicolò Gualandi, Ylenia Cortolezzis, Raffaella Picco, Monica Colitti, Francesca D'Este, Mariachiara Gani, Wayne W. Hancock, Giovanni Terrosu, Cristina Degrassi, Francesca Agostini, Claudio Brancolini, Luigi E. Xodo, Eros Di Giorgio   +13 more
wiley   +1 more source

Medial external fixation for staged treatment of closed calcaneus fractures: Surgical technique and case series

Journal of Orthopaedic Surgery, 2017
The derangement in calcaneal morphology after a fracture can be significant and is often associated with severe soft tissue envelop problems. Medial calcaneal external fixation is useful for early restoration of calcaneal morphology and the corresponding
Michael Githens, Joshua Shatsky, Julie Agel, Richard J Bransford, Stephen K Benirschke   +4 more
doaj   +1 more source

Effect of four plant species on soil 15N-access and herbage yield in temporary agricultural grasslands [PDF]

, 2013
Positive plant diversity-productivity relationships have been reported for experimental semi-natural grasslands (Cardinale et al. 2006; Hector et al. 1999; Tilman et al. 1996) as well as temporary agricultural grasslands (Frankow-Lindberg et al.
A Hector, A Hector, A Jumpponen, AH Fitter, BE Frankow-Lindberg, BJ Cardinale, C Palmborg, C Roscher, D Nyfeler, D Nyfeler, D Tilman, D Tilman, DC Whitehead, DS Powlson, F Paynel, F Wichern, G Carlsson, G Hardarson, G Hardarson, H Høgh-Jensen, H Høgh-Jensen, H. Høgh-Jensen, HE Brown, HJ Boeck De, HL Kristensen, J Rasmussen, J Ruijven van, J. Eriksen, J. Rasmussen, J. Rasmussen, K Pirhofer-Walzl, K Thorup-Kristensen, K. Pirhofer-Walzl, K. Søegaard, KJ Moore, KM Pollock, L Kirwan, L Kutschera, L Kutschera, L Mommer, M Braun, M Fried, M Scherer-Lorenzen, MA Huston, MB Peoples, PM Chalk, RH Skinner, S Felten von, S Felten von, S Felten von, S Naeem, SA Schnitzer, TE Crews, VD Picasso   +53 more
core   +1 more source

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

Controlled Release of Vancomycin and Tigecycline from an Orthopaedic Implant Coating Prevents Staphylococcus aureus Infection in an Open Fracture Animal Model. [PDF]

, 2019
Introduction:Treatment of open fractures routinely involves multiple surgeries and delayed definitive fracture fixation because of concern for infection.
Bernthal, NM, Hegde, V, Loftin, AH, Niska, J, Segura, T, Stavrakis, AI, Weixian, X, Zhu, S   +7 more
core  

Surgical technique: Tscherne-Johnson extensile approach for tibial plateau fractures. [PDF]

, 2013
BackgroundThe standard approach to lateral tibial plateau fractures involves elevation of the iliotibial band (IT) and anterior tibialis origin in continuity from Gerdy's tubercle and metaphyseal flare.
Johnson, Eric E, Osuji, Chukwunenye, Timon, Stephen   +2 more
core   +1 more source