Results 1 to 10 of about 28,220 (244)

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants [PDF]

Frontiers in Plant Science, 2021
As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects.
Gongyao Shi, Gongyao Shi, Mengyuan Hao, Mengyuan Hao, Baoming Tian, Baoming Tian, Gangqiang Cao, Gangqiang Cao, Fang Wei, Fang Wei, Zhengqing Xie, Zhengqing Xie   +11 more
doaj   +3 more sources

Function verification of a chlorophyll a/b binding protein gene through a newly established tobacco rattle virus-induced gene silencing system in Kandelia obovata [PDF]

Frontiers in Plant Science, 2023
As an important mangrove species, Kandelia obovata plays an irreplaceable role in the coastal ecosystem. However, due to a lack of genetic technology, there is limited research on its functional genes.
Mingxiong Zhang, Mingxiong Zhang, Yuhui Rao, Xiaofeng Chen, Yunrui Shi, Chonglong Wei, Xianfeng Wang, Lu Wang, Lu Wang, Chengjin Xie, Chengjin Xie, Chenglang Pan, Chenglang Pan, Jianming Chen, Jianming Chen   +14 more
doaj   +3 more sources

Establishment and optimization of a tobacco rattle virus -based virus-induced gene Silencing in Atriplex canescens [PDF]

Plant Methods
Atriplex canescens, a halophytic model plant exhibiting remarkable tolerance to abiotic stress, possesses valuable genetic resources for crop improvement.
Shan Feng, Jin-Da Chen, Ai-Ke Bao
doaj   +3 more sources

A novel gene silencing strategy based on tobacco rattle virus in Hibiscus mutabilis [PDF]

PeerJ
Background Hibiscus mutabilis L. is a popular regional characteristic plant in China, cultivated for its attractive flower colors, extended bloom time, and medicinal properties.
Shiye Sang, Yiqiong Liu, Xiu Li, Jiao Ma, Xiaoli Liu, Yuanzhao Yang   +5 more
doaj   +4 more sources

Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus [PDF]

PeerJ, 2019
Background Hibiscus hamabo Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. The genetic transformation H.
Zhiquan Wang, Xiaoyang Xu, Longjie Ni, Jinbo Guo, Chunsun Gu   +4 more
doaj   +4 more sources

Characterization of an unusual tobacco rattle virus isolate and a novel phenuivirid in the Jerusalem sage [PDF]

Virology Journal
Background The Jerusalem sage (Phlomis fruticosa) is a popular ornamental in Europe. In 2022, typical virus-like symptoms consisting of chlorotic rings and irregular patches were identified on leaves of this plant species in Lausanne, Switzerland ...
Mathieu Mahillon, Nathalie Dubuis, Justine Brodard, Isabelle Kellenberger, Arnaud G. Blouin, Olivier Schumpp   +5 more
doaj   +3 more sources

Tobacco Rattle Virus as a Tool for Rapid Reverse-Genetics Screens and Analysis of Gene Function in Cannabis sativa L. [PDF]

Plants (Basel), 2022
Medical cannabis (Cannabis sativa L.) is quickly becoming a central agricultural crop as its production has continued to increase globally. The recent release of the cannabis reference genomes provides key genetic information for the functional analysis ...
Alter H, Peer R, Dombrovsky A, Flaishman M, Spitzer-Rimon B.   +4 more
europepmc   +2 more sources

Silencing of a mannitol transport gene in Phelipanche aegyptiaca by the tobacco rattle virus system reduces the parasite germination on the host root. [PDF]

Plant Signal Behav, 2022
Root parasitic weed Phelipanche aegyptiaca is an obligate plant parasite that causes severe damage to host crops. Agriculture crops mainly belong to the Brassicaceae, Leguminosae, Cruciferae, and Solanaceae plant families affected by this parasitic weed,
Bari VK, Singh D, Nassar JA, Aly R.
europepmc   +2 more sources

Immunocapture of dsRNA-bound proteins provides insight into Tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector. [PDF]

Plant Cell, 2021
Plant RNA viruses form organized membrane-bound replication complexes to replicate their genomes. This process requires virus- and host-encoded proteins and leads to the production of double-stranded RNA (dsRNA) replication intermediates.
Incarbone M, Clavel M, Monsion B, Kuhn L, Scheer H, Vantard É, Poignavent V, Dunoyer P, Genschik P, Ritzenthaler C.   +9 more
europepmc   +2 more sources

Tobacco rattle virus-based virus-induced gene silencing (VIGS) as an aid for functional genomics in Saffron (<i>Crocus sativus</i> L.). [PDF]

Physiol Mol Biol Plants
Several limitations in genetic engineering interventions in saffron exist, hindering the development of genetically modified varieties and the widespread application of genetic engineering in this crop. Lack of genome sequence information, the complexity
Kalia D, Jose-Santhi J, Sheikh FR, Singh D, Singh RK.   +4 more
europepmc   +2 more sources