Results 31 to 40 of about 2,811 (171)

Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus

Plant Methods, 2008
Background Virus-induced gene silencing (VIGS) has emerged as a method for performing rapid loss-of-function experiments in plants. Despite its expanding use, the effect of host gene insert length and other properties on silencing efficiency have not ...
Page Jonathan E, Liu Enwu
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Downregulation of Light-Harvesting Complex II Induces ROS-Mediated Defense Against Turnip Mosaic Virus Infection in Nicotiana benthamiana

Frontiers in Microbiology, 2021
The light-harvesting chlorophyll a/b complex protein 3 (LHCB3) of photosystem II plays important roles distributing the excitation energy and modulating the rate of state transition and stomatal response to abscisic acid.
Shiyou Qiu, Shiyou Qiu, Shiyou Qiu, Xuwei Chen, Yushan Zhai, Yushan Zhai, Weijun Cui, Weijun Cui, Xuhong Ai, Xuhong Ai, Xuhong Ai, Shaofei Rao, Jianping Chen, Jianping Chen, Jianping Chen, Fei Yan, Fei Yan   +16 more
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Sequential Reconstitution of Tobacco Rattle Virus

Journal of General Virology, 1973
Summary Conditions suitable for in vitro reconstitution of the CAM strain of TRV have been defined, and various steps of rod morphogenesis have been studied. The CAM strain of TRV was found to reconstitute best at pH 4·7 in 0·5 m-phosphate buffer at low temperature, unlike the C isolate of TRV which was shown by Semancik & Reynolds (1969) to ...
M. A. Haidar, P. Pfeiffer, C. Fritsch, L. Hirth   +3 more
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Establishment and Verification of An Efficient Virus-induced Gene Silencing System in Forsythia

Horticultural Plant Journal, 2021
To understand the functional identification of large-scale genomic sequences in Forsythia, tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS), suitable for the plant, was explored in this study.
Jianshuang Shen, Weijia Si, Yutong Wu, Yang Xu, Jia Wang, Tangren Cheng, Qixiang Zhang, Huitang Pan   +7 more
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Susceptibility of transgenic tobacco plants expressing tobacco rattle virus coat protein to nematode-transmitted and mechanically inoculated tobacco rattle virus

Journal of General Virology, 1993
Transgenic Samsun NN tobacco plants expressing the coat protein of tobacco rattle virus were exposed to mechanical leaf inoculation with tobacco rattle virus and to viruliferous trichodorid vector nematodes. Whereas plants were resistant to mechanical inoculation the vector nematodes successfully transmitted tobacco rattle virus to the roots as well as
A T, Ploeg, A, Mathis, J F, Bol, D J, Brown, D J, Robinson   +4 more
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Tobacco Rattle Virus in Tobacco Mesophyll Protoplasts: Infection and Virus Multiplication

Journal of General Virology, 1975
Summary Poly-l-ornithine greatly increased infection of protoplasts by tobacco rattle virus (TRV), and had the largest effect when incubated with virus particles for at least 10 min before inoculation. Using a final concentration of 1 µg/ml TRV particles and 1 to 1.5 µg/ml poly-l-ornithine in 0.025 m-phosphate buffer, pH 6.0, to inoculate mesophyll ...
S. Kubo, B. D. Harrison, D. J. Robinson, M. A. Mayo   +3 more
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Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3

Horticultural Plant Journal, 2021
Virus-induced gene silencing (VIGS) is a natural defense mechanism of plants, which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome.
Ping Zhou, Jiayu Peng, Minjie Zeng, Lixuan Wu, Yuxin Fan, Lihui Zeng   +5 more
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Molecular and Biochemical Examination of Spraing Disease in Potato Tuber in Response to Tobacco rattle virus Infection

Molecular Plant-Microbe Interactions, 2016
Field-grown tubers of potato were examined for infection by Tobacco rattle virus (TRV) and consequent production of corky ringspot or spraing symptoms. A microarray study identified genes that are differentially expressed in tuber tissue in response to ...
Ghulam Sahi, Pete E. Hedley, Jenny Morris, Gary J. Loake, Stuart A. MacFarlane   +4 more
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A rapid, simple, and highly efficient method for VIGS and in vitro-inoculation of plant virus by INABS applied to crops that develop axillary buds and can survive from cuttings

BMC Plant Biology, 2021
Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants.
Qili Liu, Kedong Xu, Lun Yi, Yalin Hou, Dongxiao Li, Haiyan Hu, Feng Zhou, Puwen Song, Yongang Yu, Qichao Wei, Yuanyuan Guan, Ping Hu, Ruifang Bu, Eryong Chen, Xiaojia Su, Honglian Li, Chengwei Li   +16 more
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Separation and Properties of Particles of Tobacco Rattle Virus with Different Lengths [PDF]

Journal of General Microbiology, 1959
SUMMARY: Tobacco rattle virus was isolated from tobacco sap by differential centrifugation; the yield was about 50 mg. virus/1. sap. Purified preparations were highly infective, precipitated optimally at pH 4·0–4·5, and contained one electrophoretic component; chemical analysis and the ultraviolet absorption spectrum suggest they contain about 5 ...
Harrison, B. D., Nixon, H. L.
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