Gene silencing in plants by artificial small RNAs derived from minimal precursors and expressed via tobacco rattle virus. [PDF]
Highly specific, second-generation RNA interference tools are based on artificial small RNAs (art-sRNAs), such as artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs). Recent progress includes the use of minimal-
Juárez-Molina M +5 more
europepmc +2 more sources
LreEF1A4, a Translation Elongation Factor from Lilium regale, Is Pivotal for Cucumber Mosaic Virus and Tobacco Rattle Virus Infections and Tolerance to Salt and Drought. [PDF]
Eukaryotic translation elongation factors are implicated in protein synthesis across different living organisms, but their biological functions in the pathogenesis of cucumber mosaic virus (CMV) and tobacco rattle virus (TRV) infections are poorly ...
Sun D +7 more
europepmc +2 more sources
Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet. [PDF]
Corky ringspot (CRS) is a widespread potato tuber necrotic disease caused by Tobacco rattle virus (TRV) infection. In the Pacific Northwest, this virus is transmitted by the stubby root nematode (SRN) within the genus Paratrichodorus.
Quick RA +5 more
europepmc +2 more sources
Crosstalk between epigenetic silencing and infection by tobacco rattle virus in Arabidopsis. [PDF]
Summary DNA methylation is an important epigenetic mechanism for controlling innate immunity against microbial pathogens in plants. Little is known, however, about the manner in which viral infections interact with DNA methylation pathways.
Diezma-Navas L +6 more
europepmc +2 more sources
Agrobacterium-Mediated Gene Transient Overexpression and Tobacco Rattle Virus (TRV)-Based Gene Silencing in Cassava. [PDF]
Agrobacterium-mediated transient expression and virus-induced gene silencing (VIGS) are very useful in functional genomics in plants. However, whether these methods are effective in cassava (Manihot esculenta), one of the most important tropical crops ...
Zeng H +5 more
europepmc +2 more sources
Tobacco rattle virus -induced gene silencing in Hevea brasiliensis [PDF]
Abstract BackgroundSince it is very difficult to obtain gene knockouts in rubber tree (Hevea Brasiliensis) due to low genetic transformation efficiency. Virus-induced gene silencing (VIGS) is a powerful gene silencing tool that has been intensively applied in plant.
Hui-Liang Li +5 more
openaire +4 more sources
Incidence and Detection of Three Viruses (Cucumber Mosaic Virus, Lily Mottle Virus, and Tobacco Rattle Virus) in Daylily Plants Cultivated in Korea [PDF]
Daylilies (Hemerocallis spp.) are native to Northeast Asia, including Korea, China, and Japan, and are now widely cultivated as ornamental plants. This study investigated the occurrence of viruses in daylily cultivars at the National Arboretum in Pocheon.
Hae Min Lee +8 more
doaj +2 more sources
Tobacco Rattle Virus-induced Phytoene Desaturase (PDS) Silencing in Centaurea cyanus
Virus-induced gene silencing (VIGS) is a genetic tool used to assess gene function. Tobacco rattle virus (TRV) is a VIGS vector commonly used to induce endogenous gene silencing in plants.
Chengyan Deng +5 more
doaj +2 more sources
The Heterologous Expression of the p22 RNA Silencing Suppressor of the Crinivirus Tomato Chlorosis Virus from Tobacco Rattle Virus and Potato Virus X Enhances Disease Severity but Does Not Complement Suppressor-Defective Mutant Viruses [PDF]
To counteract host antiviral RNA silencing, plant viruses express suppressor proteins that function as pathogenicity enhancers. The genome of the Tomato chlorosis virus (ToCV) (genus Crinivirus, family Closteroviridae) encodes an RNA silencing suppressor,
Yazmín Landeo-Ríos +3 more
doaj +2 more sources
Comprehensive transcriptome analyses reveal tomato plant responses to tobacco rattle virus-based gene silencing vectors. [PDF]
In plants, virus-induced gene silencing (VIGS) is a popular tool for functional genomic studies or rapidly assessing individual gene functions. However, molecular details regarding plant responses to viral vectors remain elusive, which may complicate ...
Zheng Y, Ding B, Fei Z, Wang Y.
europepmc +2 more sources

