Tobacco rattle virus-induced PHYTOENE DESATURASE (PDS) and Mg-chelatase H subunit (ChlH) gene silencing in Solanum pseudocapsicum L. [PDF]
PeerJ, 2018 Virus-induced gene silencing (VIGS) is an attractive tool for determining gene function in plants. The present study constitutes the first application of VIGS in S. pseudocapsicum, which has great ornamental and pharmaceutical value, using tobacco rattle
Xu H +7 more
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Vacuum and Co-cultivation Agroinfiltration of (Germinated) Seeds Results in Tobacco Rattle Virus (TRV) Mediated Whole-Plant Virus-Induced Gene Silencing (VIGS) in Wheat and Maize. [PDF]
Front Plant Sci, 2017 Tobacco rattle virus (TRV)-mediated virus-induced gene silencing (VIGS) has been frequently used in dicots. Here we show that it can also be used in monocots, by presenting a system involving use of a novel infiltration solution (containing ...
Zhang J +11 more
europepmc +2 more sources
Efficient gene silencing mediated by tobacco rattle virus in an emerging model plant physalis. [PDF]
PLoS ONE, 2014 The fruit of Physalis has a berry and a novelty called inflated calyx syndrome (ICS, also named the 'Chinese lantern'). Elucidation of the underlying developmental mechanisms of fruit diversity demands an efficient gene functional inference platform ...
Ji-Si Zhang +3 more
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TOBACCO RATTLE VIRUS ISOLATES USING COMPLEX DIAGNOSTICS
Potato Growing, 2020 The experimental data on the search for isolates of the tobacco rattle virus using complex diagnostics of the research potatoes material is presented in the article. As a result of this work, the indicator-plant N. Debneyi was identified, which carries an isolate of tobacco rattle virus.
YU. A. HALIMONENKO +4 more
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Tobacco rattle virus infection alters Arabidopsis gene expression via virus-derived SiRNAs and host MiRNA network disruption [PDF]
BMC GenomicsBackground Virus-induced gene silencing (VIGS) technology based on tobacco rattle virus (TRV) has been widely employed in plant gene function research.
Lan Yu +5 more
doaj +2 more sources
Protocol for triggering plant gene silencing by viral delivery of short RNA [PDF]
STAR ProtocolsSummary: Virus-induced gene silencing (VIGS) leverages viral vectors to deliver 200–400-nt inserts targeting specific genes. Here, we present a protocol for triggering gene silencing by virus-delivered short RNA inserts (vsRNAi).
Arcadio García +4 more
doaj +2 more sources
Horticultural Plant Journal, 2019 Gene function analysis is challenging for Lilium due to the lack of an efficient method for stable genetic transformation. Virus-induced gene silencing (VIGS) is an attractive tool for determining gene function in plants. This study reported that Tobacco
Hua Xu +8 more
doaj +2 more sources
Transporters of trouble: trichodorids and tobacco rattle virus in potatoes.
Integrated nematode management: state-of-the-art and visions for the future, 2021 Trichodorids may cause both direct damage to the plants as well as indirect damage through transmission of Tobacco rattle virus in potatoes. Trichodorids include nematodes of among others the genera Paratrichodorus and Trichodorus, which are the most common in north-western Europe.
Brinkman, Pella, Hallmann, Johannes
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Ubiquitin-Conjugating Enzyme Positively Regulates Salicylic Acid and Jasmonic Acid Biosynthesis to Confer Broad-Spectrum Antiviral Resistance in Nicotiana benthamiana [PDF]
PlantsUbiquitin-conjugating enzyme (UBC) plays a significant role in plant hormone signal transduction. In this study, we observed that TuMV infection markedly upregulates UBC mRNA expression, suggesting a close association between UBC and viral infection ...
Xianglong Zhang +6 more
doaj +2 more sources
Virus-Induced Gene Silencing in the Tea Plant (Camellia sinensis)
Plants, 2023 The recent availability of a number of tea plant genomes has sparked substantial interest in using reverse genetics to explore gene function in tea (Camellia sinensis).
Wei Yang +6 more
doaj +1 more source