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Chip-based Total Internal Reflection Fluorescence Microscopy
Frontiers in Optics / Laser Science, 2018In present study we have performed total internal reflection fluorescence microscopy on optical waveguide chip. This enables large field of view of imaging with high axial solution and signal to noise ratio with low phototoxicity.
Balpreet S Ahluwalia +2 more
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Sub‐100‐nanometre resolution in total internal reflection fluorescence microscopy
Journal of Microscopy, 2008 SummaryCombining total internal reflection fluorescence microscopy with structured illumination allows optical wide‐field imaging with sub‐100‐nanometre resolution. We present a novel objective‐launch set‐up for standing wave illumination that takes advantage of a tunable transmission diffraction grating and transparent phase shifters actuated by ...
Beck M, Aschwanden M, Stemmer A
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Even illumination in total internal reflection fluorescence microscopy using laser light
Microscopy Research and Technique, 2008 In modern fluorescence microscopy, lasers are a widely used source of light, both for imaging in total internal reflection and epi-illumination modes. In wide-field imaging, scattering of highly coherent laser light due to imperfections in the light path
Reto Fiolka, Helge Ewers
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Optics Letters, 2008 In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence ...
Reto Fiolka, Andreas Stemmer
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Total internal reflection fluorescent microscopy
Journal of Microscopy, 1983SUMMARYThis review discusses applications of fluorescence microscopy using totally internally reflected excitation light. When totally internally reflected in a transparent solid at its interface with liquid, the excitation light beam penetrates only a short distance into the liquid. This surface electromagnetic field, called the ‘evanescent wave’, can
D, Axelrod +2 more
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Total internal reflection fluorescence microscopy of fungi
Fungal Biology Reviews, 2010Abstract A large number of critical molecular and cellular events occur at the cell surface and cortex; such as cell adhesion, secretion, cytoskeletal interactions, membrane dynamics and cell wall deposition. Investigating such phenomena using many epifluorescence microscopy methods has been challenging due to less than desirable temporal resolution,
Maho Uchida +2 more
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Scanning total internal reflection fluorescence microscopy
SPIE Proceedings, 2003Abstract not reproduced here by request of the publisher. The text is available from: http://dx.doi.org/10.1117/12.529180.
Gu, Min, Chon, James
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Total Internal Reflection Fluorescence Microscopy
2001Total internal reflection fluorescence microscopy (TIRFM) is used in cell biology for a wide range of applications to study the position and dynamics of molecules and organelles in living culture cells near the contact regions with the glass coverslip. TIRFM selectively illuminates fluorophores only in a very thin (less than 100 nm deep) layer near the
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Combined scanning probe and total internal reflection fluorescence microscopy
Methods, 2008Combining scanning probe and optical microscopy represents a powerful approach for investigating structure-function relationships and dynamics of biomolecules and biomolecular assemblies, often in situ and in real-time. This platform technology allows us to obtain three-dimensional images of individual molecules with nanometer resolution, while ...
John, Oreopoulos, Christopher M, Yip
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Field Portable Total Internal Reflection Fluorescence Microscopy
2021Herein, we report the design and development of a field portable total internal reflection fluorescence (TIRF) microscopic system. Firstly, we developed a prism based TIRF microscope which is bulky and expensive. In order to make system compact and field portable a wave guide based TIRF (wTIRF) device is then developed.
Anuj Saxena +4 more
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