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Tuning fluorophore excitation in a total-internal-reflection-fluorescence microscopy
Applied Optics, 2019In a total-internal-reflection-fluorescence-microscopy method, there is anisotropy in the polarized evanescent wave. Since the evanescent wave is used as an excitation field, the mentioned anisotropy is a disadvantage in using the total-internal-reflection-fluorescence-microscopy technique. Therefore, by theoretical and analytical approaches, and based
Elham, Sheykhi +4 more
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Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy
2011Total internal reflection fluorescence microscopy (TIRF-M) has become an increasingly popular tool to study events in close proximity to the cell cortex, such as cell adhesion (Axelrod, J Cell Biol 89:141-145, 1981; Gingell et al., J Cell Biol 100:1334-1338, 1985; Patel et al., J Cell Sci 121:1159-1164, 2008), actin (Bretschneider et al., Curr Biol 14 ...
Juliane P, Schwarz +2 more
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Quantifying Endothelial Transcytosis with Total Internal Reflection Fluorescence Microscopy (TIRF)
2022Apical-to-basal transcytosis by endothelial cells can be visualized and quantified using total internal reflection fluorescence (TIRF) microscopy of the basal membrane. Past techniques to study transcytosis including electron microscopy and transwells have several limitations such as confounding from paracellular leakage, low transfection efficiency ...
Erika, Jang +2 more
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Chapter 7 Total Internal Reflection Fluorescence Microscopy
2008Total internal reflection fluorescence microscopy (TIRFM), also known as evanescent wave microscopy, is used in a wide range of applications, particularly to view single molecules attached to planar surfaces and to study the position and dynamics of molecules and organelles in living culture cells near the contact regions with the glass coverslip ...
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Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
2005Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at the single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience.
Tetsuichi, Wazawa, Masahiro, Ueda
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Compensation of intensity loss with time in total internal reflection fluorescence microscopy
2011 4th International Conference on Biomedical Engineering and Informatics (BMEI), 2011Image stacks acquired by TIRF microscopy system suffer intensity loss due to photobleaching with time. In this paper, a new attenuation compensation method for TIRF microscopy images is proposed. Loss of intensity is assumed to vary only along time and governed by an exponential decay law and the decay process is then modeled as a decay function.
Xiangping Wu 0002 +3 more
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Alignment and Calibration of Total Internal Reflection Fluorescence Microscopy Systems
Cold Spring Harbor Protocols, 2012Live cell fluorescent microscopy is important in elucidating dynamic cellular processes such as cell signaling, membrane trafficking, and cytoskeleton remodeling. Often, transient intermediate states are revealed only when imaged and quantitated at the single-molecule, vesicle, or organelle level.
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