A Thin Layer Imaging with the Total Internal Reflection Fluorescence Microscopy [PDF]
Journal of Optoelectronical Nanostructures, 2017 Total internal reflection fluorescence microscopy (TIRFM) is an optical technique that allows imaging of a thin layer of the sample with a thickness of about 100-200 nm.
Neda Roostaie +5 more
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Total Internal Reflection Fluorescence Microscopy in Cell Biology [PDF]
Traffic, 2001 Key events in cellular trafficking occur at the cell surface, and it is desirable to visualize these events without interference from other regions deeper within. This review describes a microscopy technique based on total internal reflection fluorescence which is well suited for optical sectioning at cell‐substrate regions with an unusually thin ...
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Image contrast restoration in total internal reflection fluorescence microscopy
Results in OpticsBlurring is a fundamental constraint in images of an optical imaging system. It appears because of interactions of light with the applied optical system and therefore, deblurring depends on features of the optical imaging system. We applied the iterative
Samaneh Pahlevani +2 more
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Optical Microscopy and the Extracellular Matrix Structure: A Review
Cells, 2021 Biological tissues are not uniquely composed of cells. A substantial part of their volume is extracellular space, which is primarily filled by an intricate network of macromolecules constituting the extracellular matrix (ECM).
Joshua J. A. Poole +1 more
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Single-molecule three-color FRET with both negligible spectral overlap and long observation time. [PDF]
PLoS ONE, 2010 Full understanding of complex biological interactions frequently requires multi-color detection capability in doing single-molecule fluorescence resonance energy transfer (FRET) experiments.
Sanghwa Lee, Jinwoo Lee, Sungchul Hohng
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Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly [PDF]
, 2012 Advances in fluorescence methodologies make it possible to investigate biological systems in unprecedented detail. Over the last few years, quantitative live-cell imaging has increasingly been used to study the dynamic interactions of viruses with cells ...
Barbara Müller +5 more
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Conformation of the nuclear pore in living cells is modulated by transport state
eLife, 2020 While the static structure of the nuclear pore complex (NPC) continues to be refined with cryo-EM and x-ray crystallography, in vivo conformational changes of the NPC remain under-explored.
Joan Pulupa +3 more
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FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
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Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source
, 2009 International audienceWe present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source.
Blandin, Pierre +7 more
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3D printed miniature attenuated total internal reflection assisted fluorescence microscopy
Scientific ReportsImaging and sensing technologies are crucial in various fields, encompassing applications in cell and tissue analysis, DNA and RNA characterization, food and drug composition analysis, and forensic science. Instead of using complex and heavy conventional
Dong Hee Park +5 more
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