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Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host
Viruses, 2021 This review highlights the significant observations of human immunodeficiency virus (HIV) assembly, release and maturation made possible with advanced light microscopy techniques.
Saveez Saffarian
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Partial internal reflections on total internal reflection fluorescent microscopy [PDF]
Trends in Cell Biology, 2009 Microscopy, especially fluorescence microscopy, has proven to be a powerful method for studying biological processes. Unfortunately, some of the same features that make biological membranes powerful (for example, all of the action taking place across a narrow 4nm film) also make it difficult to visualize by fluorescence.
openaire +2 more sources
Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
, 2012 Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard +5 more
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New light on ion channel imaging by total internal reflection fluorescence (TIRF) microscopy
Journal of Pharmacological Sciences, 2015 Ion channels play pivotal roles in a wide variety of cellular functions; therefore, their physiological characteristics, pharmacological responses, and molecular structures have been extensively investigated.
Hisao Yamamura +2 more
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Dynamics of HIV-1 assembly and release. [PDF]
, 2009 Assembly and release of human immunodeficiency virus (HIV) occur at the plasma membrane of infected cells and are driven by the Gag polyprotein. Previous studies analyzed viral morphogenesis using biochemical methods and static images, while dynamic and ...
Kräusslich Hans-Georg +28 more
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Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation [PDF]
The Ukrainian Biochemical Journal, 2015 Fluorescent proteins are promising tools for studying intracellular signaling processes in lymphocytes. This brief review summarizes fluorescence-based imaging techniques developed in recent years and discusses new methodological advances, such as ...
G. A. Liubchenko +2 more
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Photonic-chip assisted correlative light and electron microscopy
Communications Biology, 2020 Tinguely et al. develop a photonic chip-based correlative light-electron microscopy system to generate co-registered multi-modal total internal reflection fluorescence microscopy (TIRF) and electron microscopy (EM) images of biological samples at ...
Jean-Claude Tinguely +8 more
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Nanomaterials, 2020 An urgent demand exists for the development of novel delivery systems that efficiently transport antibacterial agents across cellular membranes for the eradication of intracellular pathogens.
Paul Joyce +7 more
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Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue
Disease Models & Mechanisms, 2022 Developments in single-molecule microscopy (SMM) have enabled imaging individual proteins in biological systems, focusing on the analysis of protein mobility patterns inside cultured cells.
Radoslaw J. Gora +7 more
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Nature Communications, 2021 Achieving high axial resolution is challenging in single-molecule localization microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without ...
Alan M. Szalai +11 more
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