Results 11 to 20 of about 11,033,420 (264)
Detection efficiency in total internal reflection fluorescence microscopy [PDF]
Optics Express, 2008 We present a rapid and flexible framework for the accurate calculation of the detection efficiency of fluorescence emission in isotropic media as well as in the vicinity of dielectric or metallic interfaces. The framework accounts for the dipole characteristics of the emitted fluorescence and yields the absolute detection efficiency by taking into ...
Theo Lasser, Marcel Leutenegger
exaly +6 more sources
Total Internal Reflection Fluorescence Microscopy [PDF]
Cold Spring Harbor Protocols, 2015 The goal in fluorescence microscopy is to detect the signal of fluorescently labeled molecules with great sensitivity and minimal background noise. In epifluorescence microscopy, it is difficult to observe weak signals along the optical axis, owing to the overpowering signal from the out-of-focus particles.
Yildiz, Ahmet, Vale, Ronald D
core +9 more sources
Around-the-objective total internal reflection fluorescence microscopy. [PDF]
Appl Opt, 2009 Total internal reflection fluorescence (TIRF) microscopy uses the evanescent field on the aqueous side of a glass/aqueous interface to selectively illuminate fluorophores within approximately 100 nm of the interface. Applications of the method include epi-illumination TIRF, where the exciting light is refracted by the microscope objective to impinge on
Burghardt TP, Hipp AD, Ajtai K.
europepmc +5 more sources
Variable-angle total internal reflection fluorescence microscopy of intact cells of
Plant Methods, 2011 Background Total internal reflection fluorescence microscopy (TIRFM) is a powerful tool for observing fluorescently labeled molecules on the plasma membrane surface of animal cells.
Kim Myung K +5 more
doaj +2 more sources
Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy [PDF]
Journal of Cell Biology, 2000 Total internal reflection fluorescence microscopy has been applied to image the final stage of constitutive exocytosis, which is the fusion of single post-Golgi carriers with the plasma membrane. The use of a membrane protein tagged with green fluorescent protein allowed the kinetics of fusion to be followed with a time resolution of 30 frames/s ...
Daniel Axelrod +2 more
exaly +3 more sources
Total Internal Reflection Fluorescence (TIRF) Microscopy of Chlamydomonas Flagella [PDF]
, 2009 The eukaryotic flagellum is host to a variety of dynamic behaviors, including flagellar beating, the motility of glycoproteins in the flagellar membrane, and intraflagellar transport (IFT), the bidirectional traffic of protein particles between the flagellar base and tip.
Engel, Benjamin D. +5 more
core +7 more sources
Deconstructing the late phase of vimentin assembly by total internal reflection fluorescence microscopy (TIRFM). [PDF]
PLoS ONE, 2011 Quantitative imaging of intermediate filaments (IF) during the advanced phase of the assembly process is technically difficult, since the structures are several µm long and therefore they exceed the field of view of many electron (EM) or atomic force ...
Stefan Winheim +7 more
doaj +2 more sources
Total-Internal-Reflection-Fluorescence Microscopy for the Study of Nanobubble Dynamics [PDF]
Physical Review Letters, 2012 Nanobubbles can be observed with optical microscopy using the total-internal-reflection-fluorescence excitation. We report on total-internal-reflection-fluorescence visualization using rhodamine 6G at 5 μM concentration which results in strongly contrasting pictures.
Chan, Chon U., Ohl, Claus-Dieter
openaire +4 more sources
Imaging with total internal reflection fluorescence microscopy for the cell biologist. [PDF]
J Cell Sci, 2010 Total internal reflection fluorescence (TIRF) microscopy can be used in a wide range of cell biological applications, and is particularly well suited to analysis of the localization and dynamics of molecules and events near the plasma membrane. The TIRF excitation field decreases exponentially with distance from the cover slip on which cells are grown.
Mattheyses AL, Simon SM, Rappoport JZ.
europepmc +3 more sources
PLoS ONE, 2011 Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle ...
Edgar Gutierrez +7 more
doaj +3 more sources