Direct visualization of the reaction transformation and signal amplification in a DNA molecular machine with total internal reflection fluorescence microscopy [PDF]
Frontiers in Chemistry, 2013 In this study, as a proof of concept, the signal amplification in an artificial DNA molecular machine was directly visualized via total internal reflection fluorescence microscopy (TIRFM).
Rui eRen +4 more
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Single-shot super-resolution total internal reflection fluorescence microscopy. [PDF]
Nat Methods, 2018 Guo M +14 more
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Three basic types of fluorescence microscopy and recent improvement [PDF]
E3S Web of Conferences, 2021 Fluorescence microscopy is one of the most used imaging modalities in molecular biology and living specimens. To increase image contrast and spatial resolution, different type of fluorescence microscopy has been developed.
Wang Xinyi, Lai Yunyan
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Total Internal Reflection Fluorescence (TIRF) Microscopy [PDF]
Current Protocols in Cytometry, 2009 AbstractTotal internal reflection fluorescence (TIRF) microscopy (TIRFM) is an elegant optical technique that provides for the excitation of fluorophores in an extremely thin axial region (“optical section”). The method is based on the principle that when excitation light is totally internally reflected in a transparent solid (e.g., coverglass) at its ...
Simon Denham, Deborah Cutchey
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Frontiers in Sensors, 2022 Integrating artificial intelligence with SARS-CoV-2 diagnostics can help in the timely execution of pandemic control and monitoring plans. To improve the efficiency of the diagnostic process, this study aims to classify fluorescent images via traditional
Likun Zhang +37 more
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Characterizing the bio-functionalization of gold surface with total internal reflection fluorescence (TIRF) microscopy [PDF]
Proceedings of the Estonian Academy of Sciences, 2020 Quality of bioactive surface is crucial for achieving the required sensitivity and selectivity of biosensing systems. Numerous methods are available for the characterization of metal-coated surfaces, but only a few to test the efficacy of biomaterial ...
Robin Ehrminger +6 more
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, 2008 International audienceFRET detection using Total Internal Reflection Fluorescence Lifetime Imaging Microscopy and supercontinuum ...
Blandin, Pierre +7 more
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An economic, square-shaped flat-field illumination module for TIRF-based super-resolution microscopy
Biophysical Reports, 2022 Super-resolution microscopy allows complex biological assemblies to be observed with remarkable resolution. However, the presence of uneven Gaussian-shaped illumination hinders its use in quantitative imaging or high-throughput assays.
Jeff Y.L. Lam +8 more
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Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors [PDF]
, 2010 A scheme based on a W-shaped axicon mirror device for total-internal-reflection fluorescence microscopy (TIRFM) is presented. This approach combines the advantages of higher efficiency compared with traditional TIRFM, ad-justable illumination area, and ...
Zumbusch, Andreas +3 more
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Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
Nature Communications, 2021 Quantifying rapid and small cellular forces is a major challenge in mechanobiology. Here, the authors show a >2-fold spatially and >10-fold temporally force sampling improvement combining traction force microscopy with total internal reflection ...
Liliana Barbieri +12 more
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