Results 91 to 100 of about 8,388,417 (298)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

A micro-perspective on variation and universals [PDF]

, 2017
This paper approaches the questions raised in the workshop description surrounding universals and variation from the perspective of microvariation. Throughout this document we frequently refer to van Craenenbroeck and van Koppen (2016) (henceforth VCVK),
van Koppen, J.M., van Craenenbroeck, Jeroen, LS BZ Variatielinguistiek vh Nederlands, ILS Variation   +3 more
core  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Relating spatial patterns of stream metabolism to distributions of juveniles salmonids at the river network scale

Ecosphere, 2019
Understanding the factors that drive spatial patterns in stream ecosystem processes and the distribution of aquatic biota is important to effective management of these systems and the conservation of biota at the network scale.
Matthew J. Kaylor, Seth M. White, W. Carl Saunders, Dana R. Warren   +3 more
doaj   +1 more source

FUNWAVE‐GPU: Multiple‐GPU Acceleration of a Boussinesq‐Type Wave Model

Journal of Advances in Modeling Earth Systems, 2020
This paper documents development of a multiple‐Graphics Processing Unit (GPU) version of FUNWAVE‐Total Variation Diminishing (TVD), an open‐source model for solving the fully nonlinear Boussinesq wave equations using a high‐order TVD solver.
Ye Yuan, Fengyan Shi, James T. Kirby, Fujiang Yu   +3 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Phytoplankton community in the last undammed stretch of the Paraná River: considerations on the distance from the dam

Acta Limnologica Brasiliensia, 2017
Aim: To evaluate the influence of upstream reservoirs on the temporal and spatial variation of the phytoplankton community in the Paraná River channel and the importance of its main tributaries in reducing the oligotrophication process along this stretch
Susicley Jati, Jascieli Carla Bortolini, Geovani Arnhold Moresco, Aline Caroline Magro de Paula, Luzia Cleide Rodrigues, Patricia Iatskiu, Alfonso Pineda, Bárbara Furigo Zanco, Matheus Vieira da Silva, Yasmin Rodrigues Souza   +9 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Reflexivity in Meadow Mari: Binding and Agree [PDF]

, 2017
According to the Canonical Binding Theory (Chomsky 1981), anaphors must be bound in their local domain and pronominals must be free. The discovery of “long-distance anaphors” (e.g.
Volkova, Anna, ILS Variation
core   +2 more sources

Total variation stability and second-order accuracy at extrema

Electronic Journal of Differential Equations, 2013
It is well known that high order total variation diminishing (TVD) schemes for hyperbolic conservation laws degenerate to first-order accuracy, even at smooth extrema; hence they suffer from clipping error.
Ritesh Kumar Dubey
doaj