Results 91 to 100 of about 2,209,327 (291)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Horticultural intensification and plant-based diets of 18th century CE Waikato Māori in Aotearoa New Zealand

Nature Communications
In Aotearoa New Zealand, Māori oral histories, ethno-historical accounts, and archaeological evidence indicate that kūmara (sweet potato; Ipomoea batatas) and taro (Colocasia esculenta) horticulture were key drivers of population growth and cultural ...
Rebecca L. Kinaston, Sian Keith, Beatrice Hudson, Jonny Geber, Torsten Kleffmann, Claudine Stirling, Malcolm Reid, David Barr, Robyn Kramer, Emma Sudron, Waka Kotahi Tangata Whenua Working Group   +10 more
doaj   +1 more source

First Synthesis of Ergotamine-¹³CD₃ and Ergotaminine-¹³CD₃ from Unlabeled Ergotamine

Toxins
Ergot alkaloids (EAs) formed by Claviceps fungi are one of the most common food contaminants worldwide, affecting cereals such as rye, wheat, and barley.
Sven-Oliver Herter, Hajo Haase, Matthias Koch   +2 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Potentials of typical plant species from rewetted fenlands for the supply of strategic elements

Scientific Reports
Rewetting of peatlands requires the development of new biomass utilization pathways. The supply of strategic elements with key importance for the development of priority technologies, such as germanium (Ge), silicon (Si) and rare earth elements, from ...
Karina Michalska, Monika Heiermann, Carsten Lühr, Björn Meermann, Ralf Pecenka, Andreas Schulz, Nicole Langhammer, Susanne Theuerl, Annette Prochnow   +8 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Analysis of atmospheric spectra for trace gases [PDF]

The objective is the comprehensive analysis of high resolution atmospheric spectra recorded in the middle-infrared region to obtain simultaneous measurements of coupled parameters (gas concentrations of key trace constituents, total column amounts ...
Goldman, Aaron, Murcray, David G., Murcray, Frank J., Rinsland, Curtis P., Seals, Robert K., Jr., Smith, Mary Ann H.   +5 more
core   +1 more source

Change Impact Analysis based on Formalization of Trace Relations for Requirements [PDF]

, 2008
Evolving customer needs is one of the driving factors in software development. There is a need to analyze the impact of requirement changes in order to determine possible conflicts and design alternatives influenced by these changes.
Berg, K.G. van den, Göknil, A., Kurtev, I.   +2 more
core   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

ОБ ИЗГОТОВЛЕНИИ И ФУНКЦИОНАЛЬНОМ НАЗНАЧЕНИИ КОСТЯНЫХ И РОГОВЫХ ИЗДЕЛИЙ ЭПОХИ БРОНЗЫ ИЗ АРХЕОЛОГИЧЕСКОГО КОМПЛЕКСА ТУРГЕНЬ-II (ЖЕТЫСУ, ЮГО-ВОСТОЧНЫЙ КАЗАХСТАН) [PDF]

Материалы по археологии и истории античного и средневекового Причерноморья
Изделия из кости и рога, полученные в ходе полевых исследований на поселении эпохи бронзы археологического комплекса Тургень-II, рассматриваются как часть хозяйственных производств и бытовых промыслов древнего населения Жетысу.
Ержанова, А.Е., Горячев, А.А., Чернов, М.А., Антонов, М.А.   +3 more
doaj   +1 more source