Results 101 to 110 of about 5,722,533 (317)

Trace DNA Detection Using Diamond Dye: A Recovery Technique to Yield More DNA [PDF]

, 2023
This study aspires to find a new screening approach to trace DNA recovery techniques to yield a higher quantity of trace DNA from larger items of evidence. It takes the path of visualizing trace DNA on items of evidence with potential DNA so analysts can
Davis, Leah
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Identification of binding mechanisms in single molecule-DNA complexes [PDF]

, 2003
Eckel R, Ros R, Ros A, Wilking SD, Sewald N, Anselmetti D. Identification of binding mechanisms in single molecule-DNA complexes. Biophysical journal. 2003;85(3):1968-1973.Changes in the elastic properties of single deoxyribonucleic acid (DNA) molecules ...
Anselmetti, Dario, Wilking, Sven David, Ros, Robert, Eckel, Rainer, Sven David Wilking, Dario Anselmetti, Robert Ros, Wilking, S.D., Sewald, Norbert ; https://orcid.org/, Ra Ros, Ros, Alexandra, Sewald, Norbert, Rainer Eckel, Anselmetti, Dario ; https://orcid.org/, Y Norbert Sewald   +14 more
core   +1 more source

Environmental DNA metabarcoding reveals complex interactions between natural capital in orchards: Insights into arthropod community dynamics and foraging diversity

Environmental and Sustainability Indicators
Natural vegetation fragments in agroecosystems often support relatively diverse arthropod communities and the ecosystem services they deliver. Unfortunately, these natural fragments are not typically accounted for in farm management, partly due to the ...
Joshua H. Kestel, David L. Field, Philip W. Bateman, Nicole E. White, Christopher Swinstead, Paul Nevill   +5 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Metazoan diversity in Chilean hypersaline lakes unveiled by environmental DNA

Frontiers in Ecology and Evolution
Saline and hypersaline wetlands are biodiversity hotspots for metazoans such as aquatic invertebrates and wading birds. However, the survival of these habitats and their biota is increasingly threatened by a combination of pressures from climate change ...
Mattia Saccò, Mattia Saccò, Mattia Saccò, Matthew A. Campbell, Pablo Aguilar, Pablo Aguilar, Pablo Aguilar, Gonzalo Salazar, Gonzalo Salazar, Gonzalo Salazar, Tina E. Berry, Tina E. Berry, Matthew J. Heydenrych, Angus Lawrie, Nicole E. White, Chris Harrod, Chris Harrod, Chris Harrod, Morten E. Allentoft, Morten E. Allentoft   +19 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

The Global Trace Protocol

Global Trace is an open-source software tool that enables brands, producers, and manufacturers to track and trace products while detecting supplier risk through their entire supply chain.
Global Trace Protocol
core   +4 more sources

Chronic trace metals effects of mine tailings on estuarine assemblages revealed by environmental DNA

PeerJ, 2019
Mine tailing disasters have occurred worldwide and contemporary release of tailings of large proportions raise concerns of the chronic impacts that trace metals may have on the aquatic biodiversity.
Â. Bernardino, F. Pais, Louisi S. Oliveira, F. Gabriel, T. O. Ferreira, H. M. Queiroz, A. C. Mazzuco   +6 more
semanticscholar   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source