Results 41 to 50 of about 42,852 (256)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

An Effective Pyramid Neural Network Based on Graph-Related Attentions Structure for Fine-Grained Disease and Pest Identification in Intelligent Agriculture

Agriculture, 2023
In modern agriculture and environmental protection, effective identification of crop diseases and pests is very important for intelligent management systems and mobile computing application.
Sen Lin, Yucheng Xiu, Jianlei Kong, Chengcai Yang, Chunjiang Zhao   +4 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Traceable mixnets

Proceedings on Privacy Enhancing Technologies
We introduce the notion of traceable mixnets. In a traditional mixnet, multiple mix-servers jointly permute and decrypt a list of ciphertexts to produce a list of plaintexts, along with a proof of correctness, such that the association between individual ciphertexts and plaintexts remains completely hidden.
Prashant Agrawal, Abhinav Nakarmi, Mahabir Prasad Jhanwar, Subodh Sharma 0001, Subhashis Banerjee   +4 more
openaire   +3 more sources

Named entity recognition and coreference resolution using prompt-based generative multimodal

Complex & Intelligent Systems
Named Entity Recognition (NER) and Coreference Resolution (CR) are both of pivotal significance in the domain of natural language processing. The accurate detection of pronouns and noun phrases has been shown to have a direct impact on cross-sentence and
Qingchuan Zhang, Zexi Song, Delong Wang, Yuanyuan Cai, Mingwen Bi, Min Zuo   +5 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Cadmium exposure assessment based on food survey data in an urban area of Beijing and Chongli area of Zhangjiakou

Zhongguo shipin weisheng zazhi, 2022
ObjectiveTo provide a reference for food safety risk assessment and safety assurance for the Winter Olympics and Beijing residents， the exposure level and possible health risks of cadmium （Cd） in main foods in the surrounding areas of the 2022 Beijing ...
HAN Gaochao, JING Haiming, HAN Yitao, LI Nan, ZHANG Lei, SONG Yan, LUO Fei, HOU Yan, ZHANG Guoyan, LI Zinan, ZHANG Wenjing, QI Lijuan, DU Hongju, NIE Yanmin, TAN Zhuangsheng, NING Junyu, GAO Shan, LOU Yun, LI Guojun   +18 more
doaj   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source