Results 41 to 50 of about 34,553 (259)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

FEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure, Misaki K. Nishio, Satoru Okuda, Kohji Hotta   +3 more
wiley   +1 more source

An Effective Pyramid Neural Network Based on Graph-Related Attentions Structure for Fine-Grained Disease and Pest Identification in Intelligent Agriculture

Agriculture, 2023
In modern agriculture and environmental protection, effective identification of crop diseases and pests is very important for intelligent management systems and mobile computing application.
Sen Lin, Yucheng Xiu, Jianlei Kong, Chengcai Yang, Chunjiang Zhao   +4 more
doaj   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Named entity recognition and coreference resolution using prompt-based generative multimodal

Complex & Intelligent Systems
Named Entity Recognition (NER) and Coreference Resolution (CR) are both of pivotal significance in the domain of natural language processing. The accurate detection of pronouns and noun phrases has been shown to have a direct impact on cross-sentence and
Qingchuan Zhang, Zexi Song, Delong Wang, Yuanyuan Cai, Mingwen Bi, Min Zuo   +5 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Electrochemical immunosensor based on MWCNT-CS as a signal amplification strategy for the detection of capsaicinoids

International Journal of Electrochemical Science, 2021
We constructed an electrochemical immunosensor based on multiwalled carbon nanotube-chitosan (MWCNT-CS) for on-site detection of capsaicinoids, which are label compounds in waste oils.
Fengzhen Yang, Qingqing Yang, Jiansen Li, Qianqian Kong, Jingcheng Huang, Rui Xu, Yaodong Xiang, Yemin Guo   +7 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Cadmium exposure assessment based on food survey data in an urban area of Beijing and Chongli area of Zhangjiakou

Zhongguo shipin weisheng zazhi, 2022
ObjectiveTo provide a reference for food safety risk assessment and safety assurance for the Winter Olympics and Beijing residents， the exposure level and possible health risks of cadmium （Cd） in main foods in the surrounding areas of the 2022 Beijing ...
HAN Gaochao, JING Haiming, HAN Yitao, LI Nan, ZHANG Lei, SONG Yan, LUO Fei, HOU Yan, ZHANG Guoyan, LI Zinan, ZHANG Wenjing, QI Lijuan, DU Hongju, NIE Yanmin, TAN Zhuangsheng, NING Junyu, GAO Shan, LOU Yun, LI Guojun   +18 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source