Results 31 to 40 of about 42,393 (263)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Optimization of the Pure Pursuit algorithm based on real-time error

Alexandria Engineering Journal
To address the issues of vehicle overshoot on high-curvature paths (with a curvature radius less than 15 m) and the poor tracking performance on continuous turning paths in the traditional Pure Pursuit (PP) algorithm, this paper proposes an optimized ...
Fengyun Cao, Dengtao Wu, Yuxin Wu
doaj   +1 more source

Inhibition of cyclin‐dependent kinases 12/13 using CT7439 as a treatment for colorectal cancer with CDK12 upregulation

Molecular Oncology, EarlyView.
The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington, Divya L. Dayanidhi, Mohammad Zokaasadi, John B. Mantyh, Pelumi D. Olawuni, Gabrielle Rupprecht, Jeremy M. Force, Shannon McCall, Ashwani K. Bahl, David S. Hsu, Jason A. Somarelli   +10 more
wiley   +1 more source

Path Tracking Control Algorithm of Tractor-Implement

智慧农业, 2023
[Objective]The usual agricultural machinery navigation focuses on the tracking accuracy of the tractor, while the tracking effect of the trailed implement in the trailed agricultural vehicle is the core of the work quality.
LIU Zhiyong, WEN Changkai, XIAO Yuejin, FU Weiqiang, WANG Hao, MENG Zhijun   +5 more
doaj   +1 more source

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

Research on the Cooperative Target State Estimation and Tracking Optimization Method of Multi-UUV

Sensors, 2023
This work studied two sub-problems of the cooperative state estimation and cooperative optimization of tracking paths in multiple unmanned underwater vehicle (multi-UUV) cooperative target tracking.
Tao Chen, Qi Qi
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Research on Path Tracking Technology for Tracked Unmanned Vehicles Based on DDPG-PP

Machines
Realizing path tracking is crucial for improving the accuracy and efficiency of unmanned vehicle operations. In this paper, a path tracking hierarchical control method based on DDPG-PP is proposed to improve the path tracking accuracy of tracked unmanned
Yongjuan Zhao, Chaozhe Guo, Jiangyong Mi, Lijin Wang, Haidi Wang, Hailong Zhang   +5 more
doaj   +1 more source

Predicting and tracking internet path changes [PDF]

Proceedings of the ACM SIGCOMM 2011 conference, 2011
This paper investigates to what extent it is possible to use traceroute-style probing for accurately tracking Internet path changes. When the number of paths is large, the usual traceroute based approach misses many path changes because it probes all paths equally.
Cunha, Italo, Teixeira, Renata, Veitch, Darryl, Diot, Christophe   +3 more
openaire   +4 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source