Uncertainty-aware traction force microscopy. [PDF]
PLoS Computational BiologyTraction Force Microscopy (TFM) is a versatile tool to quantify cell-exerted forces by imaging and tracking fiduciary markers embedded in elastic substrates. The computations involved in TFM are often ill-conditioned, and data smoothing or regularization
Adithan Kandasamy +4 more
doaj +7 more sources
A primer to traction force microscopy [PDF]
Journal of Biological Chemistry, 2022 Traction force microscopy (TFM) has emerged as a versatile technique for the measurement of single-cell-generated forces. TFM has gained wide use among mechanobiology laboratories, and several variants of the original methodology have been proposed ...
Andrea Zancla +2 more
exaly +5 more sources
Measuring (biological) materials mechanics with atomic force microscopy. 5. Traction force microscopy (cell traction forces) [PDF]
Microscopy Research and Technique, 2023 Cells generate traction forces to probe the mechanical properties of the surroundings and maintain a basal equilibrium state of stress. Traction forces are also implicated in cell migration, adhesion and ECM remodeling, and alteration of these forces is ...
Andreas Weber, JOSÉ Luis Toca-Herrera
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Field Guide to Traction Force Microscopy [PDF]
Cellular and Molecular BioengineeringTraction force microscopy (TFM) is a widely used technique to measure cell contractility on compliant substrates that mimic the stiffness of human tissues.
Honesty Kim +2 more
exaly +7 more sources
napariTFM: An open-source tool for traction force microscopy and monolayer stress microscopy. [PDF]
PLoS Computational BiologyCellular force generation and transmission are fundamental processes driving cell migration, division, tissue morphogenesis, and disease progression.
Artur Ruppel +3 more
doaj +5 more sources
Combined Traction Force–Atomic Force Microscopy Measurements of Neuronal Cells
Biomimetics, 2022 In the course of the development of the nervous system, neuronal cells extend (grow) axons, which navigate over distances of the order of many cell diameters to reach target dendrites from other neurons and establish neuronal circuits.
Udathari Kumarasinghe +2 more
doaj +6 more sources
High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy [PDF]
Communications Biology, 2023 A critical requirement for studying cell mechanics is three-dimensional assessment of cellular shapes and forces with high spatiotemporal resolution. Traction force microscopy with fluorescence imaging enables the measurement of cellular forces, but it ...
Moosung Lee +7 more
doaj +3 more sources
An Open-source Python Tool for Traction Force Microscopy on Micropatterned Substrates [PDF]
Bio-ProtocolCell-generated forces play a critical role in driving and regulating complex biological processes, such as cell migration and division and cell and tissue morphogenesis in development and disease.
Artur Ruppel +2 more
doaj +3 more sources
A Versatile Three Dimensional Traction Force Microscopy Framework for Uncovering the Mechanics of Bio‐Adhesion [PDF]
Advanced ScienceThis study presents a novel, versatile traction force microscopy framework for quantifying three‐dimensional (3D) interfacial forces during bio‐adhesion by integrating in situ stereo‐digital image correlation with finite element (FE) simulation.
Yingwei Hou, Fusheng Wang, Tao Liu
doaj +4 more sources
3D Traction Force Microscopy in Biological Gels: From Single Cells to Multicellular Spheroids [PDF]
Annual Review of Biomedical EngineeringCell traction force plays a critical role in directing cellular functions, such as proliferation, migration, and differentiation. Current understanding of cell traction force is largely derived from 2D measurements where cells are plated on 2D substrates.
Mingming Wu, Susan Leggett
exaly +3 more sources