Cellogram: On-the-Fly Traction Force Microscopy [PDF]
Nano Letters, 2019 Traction force microscopy (TFM) derives maps of cell-generated forces, typically in the nanonewton range, transmitted to the extracellular environment upon actuation of complex biological processes. In traditional approaches, force rendering requires a terminal, time-consuming step of cell deadhesion to obtain a reference image. A conceptually opposite
Costanza Giampietro +2 more
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Enhancing robustness, precision, and speed of traction force microscopy with machine learning. [PDF]
Biophys J, 2023 Traction patterns of adherent cells provide important information on their interaction with the environment, cell migration or tissue patterns and morphogenesis.
Kratz FS, Möllerherm L, Kierfeld J.
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Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM) [PDF]
Nature Communications, 2021 Quantifying rapid and small cellular forces is a major challenge in mechanobiology. Here, the authors show a >2-fold spatially and >10-fold temporally force sampling improvement combining traction force microscopy with total internal reflection ...
Liliana Barbieri +12 more
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Publisher Correction: Epifluorescence-based three-dimensional traction force microscopy [PDF]
Scientific Reports, 2021 An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Lauren Hazlett +6 more
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3D traction force microscopy in human trabecular meshwork tissues: Effects of ROCK and YAP/TAZ inhibition in normal and glaucomatous tissues. [PDF]
Tissue CellPrimary open-angle glaucoma (POAG) is the leading cause of irreversible blindness worldwide, with an estimated 112 million people projected to be affected by 2040.
Karimi A +7 more
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Super-Resolved Traction Force Microscopy (STFM) [PDF]
Nano Letters, 2016 Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by ...
James Felce +2 more
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Fluctuation-Based Super-Resolution Traction Force Microscopy [PDF]
Nano Letters, 2020 Cellular mechanics play a crucial role in tissue homeostasis and are often misregulated in disease. Traction force microscopy is one of the key methods that has enabled researchers to study fundamental aspects of mechanobiology; however, traction force microscopy is limited by poor resolution. Here, we propose a simplified protocol and imaging strategy
Mitro Miihkinen +2 more
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Mapping Collective Forces of Lung Cancer Spheroids Using Traction Force Microscopy. [PDF]
Biotechnol BioengEpithelial tissues actively deform their surrounding extracellular matrix mechanically. Traction forces represent an intrinsic mechanism by which cells actively sense and adapt to their extracellular environment, which has been increasingly recognized to
Zhang Q, Chen J, Zhang Z, Liu W.
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Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy [PDF]
Nano Letters, 2019 Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied force probing technology but typically restricts itself to single-plane two-dimensional ...
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A computational bridge between traction force microscopy and tissue contraction. [PDF]
J Appl Phys, 2023 Arterial wall active mechanics are driven by resident smooth muscle cells, which respond to biological, chemical, and mechanical stimuli and activate their cytoskeletal machinery to generate contractile stresses. The cellular mechanoresponse is sensitive
Flanary SM +4 more
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