Results 11 to 20 of about 212,052 (223)
A detailed protocol for cell force measurement by traction force microscopy
Smart Materials in Medicine, 2023 Cellular traction forces (CTFs) are generated by adherent cells and involved in regulating migration, morphology, and homeostasis. Accurate measurement of CTFs is crucial for understanding fundamental biological processes such as morphogenesis ...
Man Zhang +6 more
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Astigmatic traction force microscopy (aTFM) [PDF]
Nature Communications, 2021 Quantifying rapidly progressing three-dimensional forces generated by cells remains a major challenge in mechanobiology. Here, the authors show that combining traction force microscopy with astigmatic imaging permits sensitive out-of-plane force ...
Di Li +8 more
doaj +6 more sources
Traction Force Microscopy with DNA FluoroCubes. [PDF]
LangmuirMechanical forces at the cell–substrate interface govern processes from migration to differentiation, yet mapping these forces at high spatial resolution remains challenging.
Mortazavi A +7 more
europepmc +5 more sources
TFMLAB: A MATLAB toolbox for 4D traction force microscopy [PDF]
SoftwareX, 2021 We present TFMLAB, a MATLAB software package for 4D (x;y;z;t) Traction Force Microscopy (TFM). While various TFM computational workflows are available in the literature, open-source programs that are easy to use by researchers with limited technical ...
Jorge Barrasa-Fano +5 more
doaj +7 more sources
Traction force microscopy of engineered cardiac tissues. [PDF]
PLoS ONE, 2018 Cardiac tissue development and pathology have been shown to depend sensitively on microenvironmental mechanical factors, such as extracellular matrix stiffness, in both in vivo and in vitro systems.
Francesco Silvio Pasqualini +5 more
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Traction force microscopy – Measuring the forces exerted by cells
Micron, 2021 Cells generate mechanical forces (traction forces, TFs) while interacting with the extracellular matrix or neighbouring cells. Forces are generated by both cells and extracellular matrix (ECM) and transmitted within the cell-ECM or cell-cell contacts ...
Tomasz Zielinski +2 more
exaly +4 more sources
Comparison of direct and inverse methods for 2.5D traction force microscopy. [PDF]
PLoS ONE, 2022 Essential cellular processes such as cell adhesion, migration and division strongly depend on mechanical forces. The standard method to measure cell forces is traction force microscopy (TFM) on soft elastic substrates with embedded marker beads. While in
Johannes W Blumberg, Ulrich S Schwarz
doaj +3 more sources
May the force be with your (immune) cells: an introduction to traction force microscopy in Immunology [PDF]
Frontiers in Immunology, 2022 For more than a couple of decades now, “force” has been recognized as an important physical parameter that cells employ to adapt to their microenvironment.
Farah Mustapha +6 more
doaj +4 more sources
Confocal reference free traction force microscopy [PDF]
Nature Communications, 2016 Traction force microscopy is an effective method of measuring forces between cells and their environment, but requires removing the cells to obtain a reference image.
Martin Bergert +12 more
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Double Sided Traction Force Microscopy: A Method to Confine Cells for Physiologically Relevant Force Measurements. [PDF]
Cytoskeleton (Hoboken)In this paper we describe a technique to make a confined environment of variable stiffness that is suitable for high-resolution live-cell imaging. This versatile and adaptable technique enables cell confinement between soft elastic surfaces made from ...
Caillier A, Oakes PW.
europepmc +3 more sources