Epifluorescence-based three-dimensional traction force microscopy. [PDF]
Sci Rep, 2020 AbstractWe introduce a novel method to compute three-dimensional (3D) displacements and both in-plane and out-of-plane tractions on nominally planar transparent materials using standard epifluorescence microscopy. Despite the importance of out-of-plane components to fully understanding cell behavior, epifluorescence images are generally not used for 3D
Hazlett L +6 more
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High-Resolution Traction Force Microscopy [PDF]
, 2014 Cellular forces generated by the actomyosin cytoskeleton and transmitted to the extracellular matrix (ECM) through discrete, integrin-based protein assemblies, that is, focal adhesions, are critical to developmental morphogenesis and tissue homeostasis, as well as disease progression in cancer.
Sergey V, Plotnikov +3 more
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Recent technological advancements in traction force microscopy. [PDF]
Biophys Rev, 2019 Ferrari A.
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L1-regularized reconstruction for traction force microscopy [PDF]
2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI), 2016 Traction Force Microscopy (TFM) is a technique widely used to recover cellular tractions from the deformation they cause in their surrounding substrate. Traction recovery is an ill-posed inverse problem that benefits of a regularization scheme constraining the solution.
Suñé Auñón, Alejandro +3 more
openaire +2 more sources
Regularization techniques and inverse approaches in 3D Traction Force Microscopy
International Journal of Mechanical SciencesJOSÉ A Sanz-Herrera +2 more
exaly +2 more sources
Traction force microscopy for understanding cellular mechanotransduction. [PDF]
BMB Rep, 2020 Under physiological and pathological conditions, mechanical forces generated from cells themselves or transmitted from extracellular matrix (ECM) through focal adhesions (FAs) and adherens junctions (AJs) are known to play a significant role in regulating various cell behaviors.
Hur SS +5 more
europepmc +4 more sources
A novel cell traction force microscopy to study multi-cellular system. [PDF]
PLoS Computational Biology, 2014 Traction forces exerted by adherent cells on their microenvironment can mediate many critical cellular functions. Accurate quantification of these forces is essential for mechanistic understanding of mechanotransduction. However, most existing methods of
Xin Tang +3 more
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Atomic Force Microscopy Characterization of Polyacrylamide Substrate for Traction Force Application
Solid State Phenomena, 2023 Atomic Force Microscopy (AFM) technology has ushered researchers to directly observe surface topology and the substrate mechanical properties using specialized probe.
R.S Rosário +4 more
semanticscholar +1 more source
Three-dimensional traction force microscopy: a new tool for quantifying cell-matrix interactions. [PDF]
PLoS ONE, 2011 The interactions between biochemical processes and mechanical signaling play important roles during various cellular processes such as wound healing, embryogenesis, metastasis, and cell migration.
Christian Franck +3 more
doaj +1 more source
Full L1-regularized Traction Force Microscopy over whole cells
BMC Bioinformatics, 2017 Background Traction Force Microscopy (TFM) is a widespread technique to estimate the tractions that cells exert on the surrounding substrate.
Alejandro Suñé-Auñón +5 more
doaj +1 more source