Results 71 to 80 of about 212,052 (223)

Cell Morphology and Biophysical Mechanisms-Informed Traction Force Microscopy Using Machine Learning

bioRxiv
Quantitative evaluation of cell motility is essential for understanding biological functions. Traction force microscopy (TFM) is a method for quantifying cellular traction forces.
Koki Fujiwara, Ryosuke Fujikawa, Yukihiro Suzuki, Kenta T. Suzuki, Y. Sakumura   +4 more
semanticscholar   +1 more source

Traction force microscopy for linear and nonlinear elastic materials as a parameter identification inverse problem [PDF]

Inverse Problems
Traction force microscopy (TFM) is a method widely used in biophysics and cell biology to determine forces that biological cells apply to their environment.
Gesa Sarnighausen, Tram Thi Ngoc Nguyen, Thorsten Hohage, Mangalika Sinha, Sarah Köster, Timothy A Betz, Ulrich Sebastian Schwarz, Anne Wald   +7 more
semanticscholar   +1 more source

Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential

Advanced Healthcare Materials, EarlyView.
This review examines how cellular behavior is regulated by mechanical cues transmitted through soft biomaterials, from single‐cell mechanosensing to tissue‐level adaptation. It highlights why physiological relevance, rather than model complexity alone, is critical for translational mechanobiology and introduces a scoring framework linking material ...
Mathias Polz, Manuel P. Kainz, Niklas Haider, Theresa Rienmüller, Kerstin Lenk, Rupert Kargl, Michele Terzano, Gerhard Sommer, Gerhard A. Holzapfel, Julia Fuchs   +9 more
wiley   +1 more source

A new approach for high-content traction force microscopy to characterize large cell ensembles

bioRxiv
Accurate measurements of cellular forces are important for understanding a wide range of biological processes where traction plays a major role.
Nicolas Desjardins-Lecavalier, Santiago Costantino   +1 more
semanticscholar   +1 more source

Traction microscopy to identify force modulation in subresolution adhesions [PDF]

Nature Methods, 2015
We present a reconstruction algorithm that resolves cellular tractions in diffraction-limited nascent adhesions (NAs). The enabling method is the introduction of sparsity regularization to the solution of the inverse problem, which suppresses noise without underestimating traction magnitude.
Han, Sangyoon J., Oak, Youbean, Groisman, Alex, Danuser, Gaudenz   +3 more
openaire   +5 more sources

Patient‐Derived 3D Heart‐On‐a‐Chip Model of Dilated Cardiomyopathy With Embedded Bead‐Based Mapping of Tissue Contractility

Advanced Healthcare Materials, EarlyView.
A heart‐on‐a‐chip model of dilated cardiomyopathy is developed from patient‐derived induced pluripotent stem cells. The model recapitulates key disease phenotypes and enables functional assessment through integrated bead‐based tracking and pillar deflection measurements.
Ali Mousavi, Ludovic Mouttet, Shihao Cui, Yasaman Hekmatnia, Mehran Mottahedi, Ida Derish, Naimeh Rafatian, Mark Aurousseau, Gregor Andelfinger, Renzo Cecere, Houman Savoji   +10 more
wiley   +1 more source

A novel method for quantifying traction forces on hexagonal micropatterned protein features on deformable poly-dimethyl siloxane sheets

MethodsX, 2019
Many methods exist for quantifying cellular traction forces, including traction force microscopy and microfabricated post arrays. However, these methodologies have limitations, including a requirement to remove cells to determine undeflected particle ...
Brian P. Griffin, Christopher J. Largaespada, Nicole A. Rinaldi, Christopher A. Lemmon   +3 more
doaj   +1 more source

Free Form Deformation-Based Image Registration Improves Accuracy of Traction Force Microscopy. [PDF]

PLoS ONE, 2015
Traction Force Microscopy (TFM) is a widespread method used to recover cellular tractions from the deformation that they cause in their surrounding substrate. Particle Image Velocimetry (PIV) is commonly used to quantify the substrate's deformations, due
Alvaro Jorge-Peñas, Alicia Izquierdo-Alvarez, Rocio Aguilar-Cuenca, Miguel Vicente-Manzanares, José Manuel Garcia-Aznar, Hans Van Oosterwyck, Elena M de-Juan-Pardo, Carlos Ortiz-de-Solorzano, Arrate Muñoz-Barrutia   +8 more
doaj   +1 more source

Jamming Transitions in Astrocytes and Glioblastoma Are Induced by Cell Density and Tension

Cells, 2022
Collective behavior of cells emerges from coordination of cell–cell-interactions and is important to wound healing, embryonic and tumor development. Depending on cell density and cell–cell interactions, a transition from a migratory, fluid-like unjammed ...
Urszula Hohmann, Julian Cardinal von Widdern, Chalid Ghadban, Maria Cristina Lo Giudice, Grégoire Lemahieu, Elisabetta Ada Cavalcanti-Adam, Faramarz Dehghani, Tim Hohmann   +7 more
doaj   +1 more source

Inhibition of VEGF‐Induced Angiogenesis and Vascular Leakage by Bicistronic Co‐Expression of Aflibercept and COMP‐Ang1

Advanced Healthcare Materials, EarlyView.
A bicistronic rAAV8 vector encoding Aflibercept (Afb) and COMP‐cAng1 simultaneously coordinates VEGF clearance and Tie2‐mediated vessel maturation. In a 3D angiogenesis‐on‐a‐chip model, rAAV8‐Afb/cAng1 effectively reverses pathological barrier breakdown under clinical disease‐mimicking challenges.
Bong‐Kyu Kim, Jaehong Min, Sang‐Hee Park, Subeen Shin, Sangbin Yu, Jeong‐Eun Park, Sujin Kim, Wooju Park, Jihoon Ko, Sunjong Kwon   +9 more
wiley   +1 more source