Results 61 to 70 of about 212,052 (223)
Traction Force Microscopy for Noninvasive Imaging of Cell Forces. [PDF]
Adv Exp Med Biol, 2018 The forces exerted by cells on their surroundings play an integral role in both physiological processes and disease progression. Traction force microscopy is a noninvasive technique that enables the in vitro imaging and quantification of cell forces. Utilizing expertise from a variety of disciplines, recent developments in traction force microscopy are
Mulligan JA +3 more
europepmc +4 more sources
Mechanosensitive traction force generation is regulated by the neutrophil activation state
Scientific Reports, 2023 The generation of traction forces by neutrophils regulates many crucial effector functions responsible for host defense, such as attachment, spreading, migration, phagocytosis, and NETosis.
Hadley Witt +4 more
doaj +1 more source
Validation tool for traction force microscopy
Computer Methods in Biomechanics and Biomedical Engineering, 2014 Traction force microscopy (TFM) is commonly used to estimate cells' traction forces from the deformation that they cause on their substrate. The accuracy of TFM highly depends on the computational methods used to measure the deformation of the substrate and estimate the forces, and also on the specifics of the experimental set-up.
Jorge-Penas, A. +3 more
openaire +4 more sources
Traction Force Microscopy to Study B Lymphocyte Activation [PDF]
Journal of Visualized Experiments, 2020 Traction force microscopy (TFM) enables the measurement of forces produced by a cell on a substrate. This technique infers traction force measurements from an experimentally observed displacement field produced by a cell pulling on an elastic substrate. Here, we adapted TFM to investigate the spatial and temporal structure of the force field exerted by
Pierobon, Paolo +4 more
openaire +3 more sources
Model-based traction force microscopy reveals differential tension in cellular actin bundles. [PDF]
PLoS Computational Biology, 2015 Adherent cells use forces at the cell-substrate interface to sense and respond to the physical properties of their environment. These cell forces can be measured with traction force microscopy which inverts the equations of elasticity theory to calculate
Jérôme R D Soiné +5 more
doaj +1 more source
STAR Protocols, 2022 Summary: Traction force microscopy (TFM) is a popular technique for studying cellular stresses; however, the reproducible fabrication of ultrasoft substrates for the reliable detection of weak cellular stresses (below 100 Pa) remains a challenge.
Farah Mustapha +2 more
doaj +1 more source
Data-driven traction force microscopy in 3D collagen hydrogels
bioRxivQuantitative measurements of cell-generated forces in fibrillar hydrogels have traditionally modeled the extracellular matrix as a continuum. Here we present a data-driven 3D traction force microscopy (DD-TFM) approach that reconstructs discrete collagen
Jorge Barrasa-Fano +7 more
semanticscholar +1 more source
Advanced Functional Materials, EarlyView.A swelling‐programmed micropatterned hydrogel guides adherent cells through a controlled transition from cell–matrix anchoring to cadherin‐mediated cell–cell compaction, enabling rapid assembly of high‐viability spheroids with defined size and morphology.
Han Gyeol Nam +8 more
wiley +1 more source
Molecular Counting in Traction Force Microscopy [PDF]
Biophysical Journal, 2015 The mechanical respons of the micro environment of a cell is of great influence for its differentiation and mobility. However, the mechanism that a cell uses to sense these mechanical properties remains largely unkown. Mechanosensing could happen by direct coupling to the nucleus via the actin cytoskeleton or by activating molecular signaling cascades ...
Harkes, Rolf +3 more
openaire +1 more source
Advanced Functional Materials, EarlyView.Molecular rotors are fluorescent molecules that are sensitive to viscosity and temperature variations in the environment. They exhibit a direct correlation between fluorescence and viscosity within Newtonian fluids. However, this correlation becomes less straightforward in complex systems.
Flavia Di Scala +6 more
wiley +1 more source