Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Transcription factor codes patterning neuronal groundplans of the cerebrum. [PDF]
Elkahlah NA +5 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
A New Conditional Transcription Factor for Use in Toxoplasma Gondii. [PDF]
Sharifpour MF +12 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
ETS transcription factor ELK3: Molecular insights and translational horizons in cancer (Review). [PDF]
Liu Z, Wei Z, Li Z, Zhou L.
europepmc +1 more source
Unstructured transcription factor interactions enable emergent specificity. [PDF]
Abidi AA +11 more
europepmc +1 more source
Timing it right: dephosphorylation of a key transcription factor constrains the tapetal transcriptional cascade for timely pollen development. [PDF]
González-Suárez P, Lara-Mondragón C.
europepmc +1 more source
Transcription Factor Rv1453 Contributes to Multidrug Sensitivity of <i>Mycobacterium tuberculosis</i>: A Potential Role for Lipid Metabolism. [PDF]
Zhang L +6 more
europepmc +1 more source
Lily NIN-like transcription factor LlNLP7 confers thermotolerance and promotes growth. [PDF]
Liao Z +5 more
europepmc +1 more source

