Results 11 to 20 of about 8,622 (220)
Massively Systematic Transcript End Readout, "MASTER": Transcription Start Site Selection, Transcriptional Slippage, and Transcript Yields. [PDF]
Mol Cell, 2015 We report the development of a next-generation sequencing-based technology that entails construction of a DNA library comprising up to at least 4(7) (∼ 16,000) barcoded sequences, production of RNA transcripts, and analysis of transcript ends and transcript yields (massively systematic transcript end readout, "MASTER").
Vvedenskaya IO +7 more
europepmc +6 more sources
Productive mRNA stem loop-mediated transcriptional slippage: Crucial features in common with intrinsic terminators. [PDF]
Proc Natl Acad Sci U S A, 2015 Significance Perturbation of transcription register by RNA polymerase, transcription slippage, is used to yield additional protein products. Known functionally important cases involve a small number of short sequences without secondary structure.
Penno C +9 more
europepmc +5 more sources
Looking ultra deep: Short identical sequences and transcriptional slippage
Genomics, 2011 Studying transcriptomes by ultra deep sequencing provides an in-depth picture of transcriptional regulation and it facilitates the detection of rare transcriptional events. Using ultra deep sequencing of amplicons we identified known isoforms and also various new low frequency variants.
Barbera D C Van Schaik +2 more
exaly +4 more sources
Bacteriophage λ N protein inhibits transcription slippage by Escherichia coli RNA polymerase
Nucleic Acids Research, 2014 Transcriptional slippage is a class of error in which ribonucleic acid (RNA) polymerase incorporates nucleotides out of register, with respect to the deoxyribonucleic acid (DNA) template. This phenomenon is involved in gene regulation mechanisms and in the development of diverse diseases.
Mikhail Kashlev
exaly +3 more sources
PLoS ONE, 2014 The thermal stability and topology of non-canonical structures of G-quadruplexes and hairpins in template DNA were investigated, and the effect of non-canonical structures on transcription fidelity was evaluated quantitatively.
Hisae Tateishi-Karimata +2 more
doaj +3 more sources
Transcriptional slippage in the positive-sense RNA virus family Potyviridae. [PDF]
EMBO Rep, 2015 The family Potyviridae encompasses ~30% of plant viruses and is responsible for significant economic losses worldwide. Recently, a small overlapping coding sequence, termed pipo, was found to be conserved in the genomes of all potyvirids. PIPO is expressed as part of a frameshift protein, P3N-PIPO, which is essential for virus cell-to-cell movement ...
Olspert A +4 more
europepmc +4 more sources
Propensity of a picornavirus polymerase to slip on potyvirus-derived transcriptional slippage sites. [PDF]
J Gen Virol, 2019 The substitution rates of viral polymerases have been studied extensively. However less is known about the tendency of these enzymes to ‘slip’ during RNA synthesis to produce progeny RNAs with nucleotide insertions or deletions. We recently described the functional utilization of programmed polymerase slippage in the family Potyviridae.
Stewart H, Olspert A, Butt BG, Firth AE.
europepmc +3 more sources
Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use. [PDF]
Nucleic Acids Res, 2016 Genetic decoding is not 'frozen' as was earlier thought, but dynamic. One facet of this is frameshifting that often results in synthesis of a C-terminal region encoded by a new frame. Ribosomal frameshifting is utilized for the synthesis of additional products, for regulatory purposes and for translational 'correction' of problem or 'savior' indels ...
Atkins JF +4 more
europepmc +6 more sources
The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae. [PDF]
J Biol Chem, 2013 The fidelity of RNA synthesis depends on both accurate template-mediated nucleotide selection and proper maintenance of register between template and RNA. Loss of register, or transcriptional slippage, is particularly likely on homopolymeric runs in the template.
Strathern J +8 more
europepmc +3 more sources
Use of transcriptional slippage for diverse gene expression
Acta Biochimica Polonica, 2021 We present here an alternative for two-promoter systems ensuring highly diverse expression of several genes from a single promoter. This approach assumes an introduction of a deletion mutation into an A/T homopolymeric run in a gene’s proximal part, and employs the transcriptional slippage mechanism for insertion-dependent reinstatement of the proper ...
Dawid, Koscielniak +3 more
openaire +3 more sources