Results 91 to 100 of about 776,254 (293)

Models for transcript quantification from RNA-Seq [PDF]

, 2011
RNA-Seq is rapidly becoming the standard technology for transcriptome analysis. Fundamental to many of the applications of RNA-Seq is the quantification problem, which is the accurate measurement of relative transcript abundances from the sequenced reads.
Pachter, Lior
core  

The South American fruit fly : an important pest insect with RNAi-sensitive larval stages [PDF]

, 2019
RNA interference (RNAi) technology has been used in the development of approaches for pest control. The presence of some essential genes, the so-called "core genes," in the RNAi machinery is crucial for its efficiency and robust response in gene ...
Cagliari, Deise, Dias, Nayma, Kremer, Frederico Schmitt, Nava, Dori Edson, Rickes, Leticia Neutzling, Smagghe, Guy, Zotti, Moises   +6 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Gene Expression Profile Related to the Progression of Preneoplastic Nodules toward Hepatocellular Carcinoma in Rats

Neoplasia: An International Journal for Oncology Research, 2006
In this study, we investigated the time course gene expression profile of preneoplastic nodules and hepatocellular carcinomas (HCC) to define the genes implicated in cancer progression in a resistant hepatocyte model. Tissues that included early nodules (
Julio Isael Pérez-Carréon, Cristina López-García, Samia Fattel-Fazenda, Evelia Arce-Popoca, Leticia Alemán-Lazarini, Sergio Hernández-García, Véronique Le Berre, Sergueï Sokol, Jean Marie Francois, Saúl Villa-Treviño   +9 more
doaj   +1 more source

Transcriptome and Temporal Transcriptome Analyses in Single Cells

International Journal of Molecular Sciences
Transcriptome analysis in single cells, enabled by single-cell RNA sequencing, has become a prevalent approach in biomedical research, ranging from investigations of gene regulation to the characterization of tissue organization. Over the past decade, advances in single-cell RNA sequencing technology, including its underlying chemistry, have ...
Jun Lyu, Chongyi Chen
openaire   +2 more sources

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source

Methodology of DNA extraction and sequencing from living cardiomyocytes collected by catheter in humans

Genetics in Medicine Open
Purpose: We present here the technical feasibility of percutaneously retrieving cardiomyocytes (CMs) through the lumen of irrigated ablation catheters, with the aim of obtaining DNA of sufficient quality/quantity for allowing DNA amplification, screening,
Flavie Ader, Céline Guilbeau-Frugier, Emeline Lhuillier, Frédéric Martins, Anne Alicia Gonzalez, Anne Rollin, Maxime Beneyto, Céline Gales, Filipe Pires, Jean-José Maoret, Jean-Michel Sénard, Jean Timnou-Bekouti, Eric Villard, Laetitia Duboscq-Bidot, Estelle Gandjbakhch, Philippe Maury   +15 more
doaj   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

In the era of whole transcriptome sequencing: Reflections on the Molecular Genetic Effect of Prenatal Sildenafil for Fetal Growth Restriction

Journal of Trial and Error
In this reflection article, we evaluate a sub study of the STRIDER trial by Terstappen et al., which investigated the molecular effects of prenatal sildenafil administration in pregnancies complicated by fetal growth restriction (FGR).
Carsten F.J. Bakhuis, Marcel A.G. van der Heyden   +1 more
doaj   +1 more source

Genomic repeats, misassembly and reannotation: a case study with long-read resequencing of Porphyromonas gingivalis reference strains

BMC Genomics, 2018
Background Without knowledge of their genomic sequences, it is impossible to make functional models of the bacteria that make up human and animal microbiota.
Luis Acuña-Amador, Aline Primot, Edouard Cadieu, Alain Roulet, Frédérique Barloy-Hubler   +4 more
doaj   +1 more source