Results 101 to 110 of about 602,244 (307)

In Situ Fixation Redefines Quiescence and Early Activation of Skeletal Muscle Stem Cells

Cell Reports, 2017
Summary: State of the art techniques have been developed to isolate and analyze cells from various tissues, aiming to capture their in vivo state. However, the majority of cell isolation protocols involve lengthy mechanical and enzymatic dissociation ...
Léo Machado, Joana Esteves de Lima, Odile Fabre, Caroline Proux, Rachel Legendre, Anikó Szegedi, Hugo Varet, Lars Roed Ingerslev, Romain Barrès, Frédéric Relaix, Philippos Mourikis   +10 more
doaj   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress [PDF]

, 2009
Dittami SM, Scornet D, Petit J-L, et al. Global expression analysis of the brown alga Ectocarpus siliculosus (Phaeophyceae) reveals large-scale reprogramming of the transcriptome in response to abiotic stress. Genome Biology.
Boyen, C., Rainer König, Da Silva, Corinne, König, Rainer, Tonon, Thierry, Van de Peer, Y., Ségurens, Béatrice, Thierry Tonon, Dittami, Simon M, Corre, Erwan, Boyen, Catherine, Strerck, Lieven, Simon M Dittami, Dondrup, M., Corinne Da Silva, Glatting, K.-H., Cock, J.M., Dittami, Simon, Da Silva, C., Lieven, Sterck, Corre, E., Cock, J. Marc, Tonon, T., Glatting, Karl-Heinz, Cock, J Mark, Sterck, L., Béatrice Ségurens, J Mark Cock, Van de Peer, Yves, Lieven Sterck, Cock, J. Mark, Dittami, Simon M., Rouzé, Pierre, Erwan Corre, Sterck, Lieven, Dondrup, Michael, Catherine Boyen, Petit, Jean-Louis, Delphine Scornet, Scornet, Delphine, Dittami, S.M., Segurens, B., Rouzé, P., Jean-Louis Petit, Karl-Heinz Glatting, Michael Dondrup, Pierre Rouzé, Scornet, D., König, R., Petit, J.-L., Yves Van de Peer   +50 more
core   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Transcriptome-guided MiMICS improved predictions of microscale metabolic heterogeneity and NO microenvironment in PA14 biofilm.

Shown are representative 3D renderings of PA14 biofilms grown for ten-hours from the experiment, and transcriptome-free and transcriptome-guided MiMICS simulations. Cells are colored according to their metabolic state. Shown are 2D yz slices (x = 160 μm,
Mohammad Mazharul Islam (1819852), Tracy J. Kuper (18452180), Shayn M. Peirce-Cottler (12254549), Jason A. Papin (8721624), Roseanne M Ford (18452183)   +4 more
core   +1 more source

The transcriptome of Nacobbus aberrans reveals insights into the evolution of sedentary endoparasitism in plant-parasitic nematodes [PDF]

, 2014
This work was supported by the Biotechnology and Biological Sciences Research Council and The James Hutton Institute (JHI). The James Hutton Institute receives funding from the Scottish Government.
Jones, John T., Cock, Peter J. A., Urwin, P.E., Eves-van den Akker, Sebastian, Cock, P.J.A., Danchin, Etienne, Danchin, Etienne G. J., Lilley, Catherine J., Urwin, Peter E., Jones, J.T., Lilley, C.J., Eves-van den Akker, S, Rancurel, Corinne   +12 more
core   +1 more source

Protein arginine methyltransferase 6 controls erythroid gene expression and differentiation of human CD34+ progenitor cells

Haematologica, 2018
Hematopoietic differentiation is driven by transcription factors, which orchestrate a finely tuned transcriptional network. At bipotential branching points lineage decisions are made, where key transcription factors initiate cell type-specific gene ...
Stefanie C. Herkt, Olga N. Kuvardina, Julia Herglotz, Lucas Schneider, Annekarin Meyer, Claudia Pommerenke, Gabriela Salinas-Riester, Erhard Seifried, Halvard Bonig, Jörn Lausen   +9 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

The DREAM complex through its subunit Lin37 cooperates with Rb to initiate quiescence

eLife, 2017
The retinoblastoma Rb protein is an important factor controlling the cell cycle. Yet, mammalian cells carrying Rb deletions are still able to arrest under growth-limiting conditions.
Christina FS Mages, Axel Wintsche, Stephan H Bernhart, Gerd A Müller   +3 more
doaj   +1 more source