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Transcriptomic Analyses of Phytoplasmas
2018Transcriptomic analyses addressed to study phytoplasma gene expression may present few difficulties due to the uncultivable nature of these intracellular, obligate pathogens. While RNA extraction from insect vectors does not imply any particular adaptation of the protocols used in most commercial kits, RNA isolation from phytoplasma-infected plants can
Pacifico D, Abba S, Palmano S
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Transcriptomics on Small Samples
2012Interrogating the cell-specific transcriptome forms an important component of understanding the role that specific cells play in assisting a plant to overcome abiotic stress. Among the challenges arising when extracting RNA from individual plant cells are: the isolation of pure cell populations; the small yield of material when isolating specific cell ...
Roy, S. +4 more
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mRNA-Seq whole-transcriptome analysis of a single cell
Nature Methods, 2009K. Lao +11 more
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Smart-seq2 for sensitive full-length transcriptome profiling in single cells
Nature Methods, 2013S. Picelli +5 more
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