Results 101 to 110 of about 585,058 (294)
Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells
Viruses(1) Recombinant protein production in mammalian cells is either based on transient transfection processes, often inefficient and underlying high batch-to-batch variability, or on laborious generation of stable cell lines.
Maria Toth +5 more
doaj +1 more source
BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma
FEBS Open Bio, EarlyView.The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru +6 more
wiley +1 more source
Insulin receptor activation and down-regulation by cationic lipid transfection reagents [PDF]
, 2004 Camilla Pramfalk +7 more
openalex +1 more source
Advanced Biology, EarlyView.The article explores the discovery of unusually large, ring‐shaped ESCRT protein structures formed by immune and stromal cells. It investigates their formation, composition, and potential roles in immune synapse formation. The findings challenge assumptions about their function in immunological synapses, suggesting an exclusive role in maintaining ...
Anthi Psoma +3 more
wiley +1 more source
Differentiation Treatment Applied to Lung Cancer Model Reduces Pathogenic Traits in Vitro
Advanced Biology, EarlyView.Non‐small cell lung cancer cells (A549) treated with a differentiation medium showed strong reduction in proliferation, migration, clonogenicity, spheroid formation and stemness markers, with enhanced adhesion properties and alveolar features, all pointing to a notably less aggressive phenotype in support of differentiation therapy as a promising ...
Alice Grossi +10 more
wiley +1 more source
Comparison of FTIR transmission and transfection substrates for canine liver cancer detection
, 2014 Kamila Kochan +6 more
openalex +2 more sources
Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells
Advanced Functional Materials, EarlyView.CRISPR machinery in diverse molecular formats (DNA, RNA, and ribonucleic protein) is complexed into nanoparticles with the cell‐friendly arginine‐alanine‐leucine‐alanine (RALA) cell‐penetrating peptide. Nanoparticles are delivered to primary mesenchymal stem cells ex vivo or locally in vivo to facilitate multimodal CRISPR gene editing. This RALA‐CRISPR
Joshua P. Graham +9 more
wiley +1 more source
Advanced Functional Materials, EarlyView.Many known diseases arise from dysregulated gene expression, and differentially expressed genes can serve as biomarkers to distinguish diseased cells from healthy tissues. In this study, reconfigurable nucleic acid nanoparticles (recNANPs) are introduced that can detect overexpressed cancer biomarkers and subsequently release RNAi inducers to silence ...
Yelixza I. Avila +7 more
wiley +1 more source