Results 101 to 110 of about 851,785 (340)

A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications. [PDF]

, 2015
RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a
Chen, Qiu-Xia, Urayama, Shiro, Wang, Wei-Peng, Yu, Ai-Ming, Zeng, Su   +4 more
core   +2 more sources

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

Evaluation of VEGF-111b Anticancer Peptide Production in Transfected Human Cells Using Real time- PCR Analysis

Journal of Mazandaran University of Medical Sciences, 2017
Background and purpose: Endothelial growth factor type b with anti-angiogenic activity and inhibition of tumor growth are considered as new anticancer drugs.
Morteza Sadeghi, Zohreh Hojati
doaj  

Investigating Gene Function for Neuronal Survival After Metabolic Stress Using Semi-Automated Fluorescence Microscopy and Automated Image Analysis

Frontiers in Molecular Neuroscience, 2018
Overexpression approaches and fluorescence microscopy techniques allow investigating important spatiotemporal aspects of gene regulation as well as quantifying gene function.
Kristin Wendland, Kristin Wendland, Andreas Meisel, Andreas Meisel, Andreas Meisel, Andreas Meisel, Philipp Mergenthaler, Philipp Mergenthaler, Philipp Mergenthaler, Philipp Mergenthaler, Philipp Mergenthaler   +10 more
doaj   +1 more source

Intrabodies Binding the Proline-Rich Domains of Mutant Huntingtin Increase Its Turnover and Reduce Neurotoxicity [PDF]

, 2008
Although expanded polyglutamine (polyQ) repeats are inherently toxic, causing at least nine neurodegenerative diseases, the protein context determines which neurons are affected.
Bugg, Charles W., Dunn, Denise E., Khoshnan, Ali, Lo, Donald C., Patterson, Paul H., Southwell, Amber L.   +5 more
core   +2 more sources

Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin

Molecular Oncology, EarlyView.
The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla, Jan Kosla, Magdalena Prechova, Lukas Frick, Patricia Bortel, Yasmin Borutzki, Andrea Bileck, Christopher Gerner, Samuel M. Meier‐Menches, Selma Osmanagic‐Myers, Martin Gregor   +10 more
wiley   +1 more source

The mechanism of high transfection efficiency of human serum albumin conjugated polyethylenimine in A549 cells

Journal of Medical Sciences, 2015
Background: In our previous study, HSA-PEI demonstrated high pDNA transfection efficiency and low cytotoxicity. Materials and Methods: In this study, the relationship between albumin receptors and the high pDNA transfection efficiency of HSA-PEI was ...
Ssu-Wei Peng, Wei-Hsu Ko, Ming-Kung Yeh, Chiao-Hsi Chiang, Jiin-Long Chen   +4 more
doaj   +1 more source

Cell cycle progression or translation control is not essential for vesicular stomatitis virus oncolysis of hepatocellular carcinoma. [PDF]

, 2010
The intrinsic oncolytic specificity of vesicular stomatitis virus (VSV) is currently being exploited to develop alternative therapeutic strategies for hepatocellular carcinoma (HCC). Identifying key regulators in diverse transduction pathways that define
A Bianchini, A Villanueva, Alexandra Junger, Antonia Rizzani, DA Altomare, EF Dunn, Enrico N. De Toni, Günter Schneider, HG Wendel, I Fabregat, I Nicoletti, IBCJ Rosenwald, JE Dancey, Jennifer Altomonte, JH Connor, JS Orlando, K Shinozaki, K Shinozaki, L Mishra, LM Schang, M Ahmed, M Hidalgo, M Ikeda, M Sun, Maciej Lesniak, N Kato, N Kato, N Kemeny, N Sonenberg, NA Hamid, Nobuyuki Kato, O Ebert, O Ebert, O Larsson, Oliver Ebert, P Chakraborty, P Newell, PC Chiang, PL Hallenbeck, RB Kjellerup, RL Silva, RM Memmott, Roland M. Schmid, S Balachandran, S Joshi, S Marozin, S Oliere, S Sei, S Sell, Sabrina Marozin, SM Steinberg, SR Kimball, T Cascone, T Nakamura, T Ueda, TL Nguyen, TP Cripe, WE Thasler, Wolfgang E. Thasler, XQ Lun, Y Ma   +60 more
core   +3 more sources

Protocol for simultaneous detection of dual subcellular localized dengue virus protease by co-transfection

, 2023
Lekha Gandhi, Musturi Venkataramana
openalex   +1 more source

Therapeutic strategies for MMAE‐resistant bladder cancer through DPP4 inhibition

Molecular Oncology, EarlyView.
We established monomethyl auristatin E (MMAE)‐resistant bladder cancer (BC) cell lines by exposure to progressively increasing concentrations of MMAE in vitro. RNA sequencing showed DPP4 expression was increased in MMAE‐resistant BC cells. Both si‐DPP4 and the DPP4 inhibitor sitagliptin suppressed the viability of MMAE‐resistant BC cells.
Gang Li, Shuichi Tatarano, Hirofumi Yoshino, Saeki Saito, Mitsuhiko Tominaga, Junya Arima, Ikumi Fukuda, Takashi Sakaguchi, Ryosuke Matsushita, Yasutoshi Yamada, Hideki Enokida   +10 more
wiley   +1 more source